Transient receptor potential melastatin-3 (TRPM3) mediates nociceptive-like responses in Hydra vulgaris

Valentina Malafoglia, Lorenzo Traversetti, Floriano Del Grosso, Massimiliano Scalici, Filomena Lauro, Valeria Russo, Tiziana Persichini, Daniela Salvemini, Vincenzo Mollace, Massimo Fini, William Raffaeli, Carolina Muscoli, Marco Colasanti

Research output: Contribution to journalArticle

Abstract

The ability of mammals to feel noxious stimuli lies in a heterogeneous group of primary somatosensory neurons termed nociceptors, which express specific membrane receptors, such as the Transient Receptor Potential (TRP) family. Here, we show that one of the most important nociceptive-like pathways is conserved in the freshwater coelenterate Hydra vulgaris, the most primitive organism possessing a nervous system. In particular, we found that H. vulgaris expresses TRPM3, a nociceptor calcium channel involved in the detection of noxious heat in mammals. Furthermore, we detected that both heat shock and TRPM3 specific agonist (i.e., pregnenolone sulfate) induce the modulation of the heat shock protein 70 (HSP70) and the nitric oxide synthase (NOS), two genes activated by TRP-mediated heat painful stimuli in mammals. As expected, these effects are inhibited by a TRPM3 antagonist (i.e., mefenamicacid). Interestingly, the TRPM3 agonist and heat shock also induce the expression of nuclear transcription erythroid 2-related factor (Nrf2) and superoxide dismutase (SOD), known markers of oxidative stress; noteworthy gene expression was also inhibited by the TRPM3 antagonist. As a whole, our results demonstrate the presence of conserved molecular oxidative/nociceptive-like pathways at the primordial level of the animal kingdom.

Original languageEnglish
Article numbere0151386
JournalPLoS One
Volume11
Issue number3
DOIs
Publication statusPublished - Mar 1 2016

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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    Malafoglia, V., Traversetti, L., Del Grosso, F., Scalici, M., Lauro, F., Russo, V., Persichini, T., Salvemini, D., Mollace, V., Fini, M., Raffaeli, W., Muscoli, C., & Colasanti, M. (2016). Transient receptor potential melastatin-3 (TRPM3) mediates nociceptive-like responses in Hydra vulgaris. PLoS One, 11(3), [e0151386]. https://doi.org/10.1371/journal.pone.0151386