Translational study searching for synergy between glycopyrronium and indacaterol

Mario Cazzola, Luigino Calzetta, Andrea Segreti, Francesco Facciolo, Paola Rogliani, Maria Gabriella Matera

Research output: Contribution to journalArticle

Abstract

We aimed to explore whether the acute bronchodilation induced by indacaterol 150 μg and glycopyrronium bromide 50 μg is additive or synergistic with respect to monocomponents by testing the type of effect ex vivo on isolated human bronchi and then in vivo in COPD patients. Both indacaterol and glycopyrronium caused a concentration-dependent relaxation of human isolated bronchial tissues sub-maximally pre-contracted with acetylcholine; glycopyrronium was significantly more potent than indacaterol. The analysis of data using the Bliss Independence (BI) criterion indicated that glycopyrronium plus indacaterol produced an additive interaction at the isoeffective concentrations inducing EC20 and a significant synergistic relaxant effect at isoeffective concentrations inducing EC30. In COPD patients, the inhalation of indacaterol and glycopyrronium in combination significantly anticipated at 15 min post-administration the mean peak of bronchodilatory effect compared to the two drugs administered alone. The study of interaction between indacaterol and glycopyrronium by BI analysis evidenced an additive effect for FEV1 between 5 min and 180 min post-inhalation, with synergistic interaction at 15 min post-administration, compared to the bronchodilation induced by these drugs administered alone. This study suggests that the combination ensures a broncholytic effect that is greater than that induced by the single monocomponents.

Original languageEnglish
Pages (from-to)175-181
Number of pages7
JournalCOPD: Journal of Chronic Obstructive Pulmonary Disease
Volume12
Issue number2
DOIs
Publication statusPublished - Apr 14 2015

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Keywords

  • Combination therapy
  • Glycopyrronium
  • Indacaterol
  • Synergy
  • Traslational evaluation

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

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