Translocation of signalling proteins to the plasma membrane revealed by a new bioluminescent procedure

Carlotta Giorgi, Anna Romagnoli, Chiara Agnoletto, Leda Bergamelli, Giovanni Sorrentino, Marisa Brini, Tullio Pozzan, Jacopo Meldolesi, Paolo Pinton, Rosario Rizzuto

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Activation by extracellular ligands of G protein-coupled (GPCRs) and tyrosine kinase receptors (RTKs), results in the generation of second messengers that in turn control specific cell functions. Further, modulation/amplification or inhibition of the initial signalling events, depend on the recruitment onto the plasma membrane of soluble protein effectors.High throughput methodologies to monitor quantitatively second messenger production, have been developed over the last years and are largely used to screen chemical libraries for drug development. On the contrary, no such high throughput methods are yet available for the other aspect of GPCRs regulation, i.e. protein translocation to the plasma membrane, despite the enormous interest of this phenomenon for the modulation of receptor downstream functions. Indeed, to date, the experimental procedures available are either inadequate or complex and expensive.Results: Here we describe the development of a novel conceptual approach to the study of cytosolic proteins translocation to the inner surface of the plasma membrane. The basis of the technique consists in: i) generating chimeras between the protein of interests and the calcium (Ca 2+)-sensitive, luminescent photo-protein, aequorin and ii) taking advantage of the large Ca 2+ concentration [Ca 2+] difference between bulk cytosolic and the sub-plasma membrane rim.Conclusion: This approach, that keeps unaffected the translocation properties of the signalling protein, can in principle be applied to any protein that, upon activation, moves from the cytosol to the plasma membrane.Thus, not only the modulation of GPCRs and RTKs can be investigated in this way, but that of all other proteins that can be recruited to the plasma membrane also independently of receptor activation.Moreover, its automated version, which can provide information about the kinetics and concentration-dependence of the process, is also applicable to high throughput screening of drugs affecting the translocation process.

Original languageEnglish
Article number27
JournalBMC Cell Biology
Volume12
DOIs
Publication statusPublished - Jun 22 2011

ASJC Scopus subject areas

  • Cell Biology

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