Abstract
To evaluate the effects of in vivo β-IFN-1b treatment on transmigration of mononuclear cells, we monitored for one year in vitro mononuclear cells trafficking through HUVECs monolayers stratified over a collagen gel during β-IFN-1b treatment of 7 RR MS patients. The number of transmigrated cells was analysed before treatment (T0) and after 3 (T3), 6 (T6) and 12 months (T12); at the same time, levels of serum MMP-9 were quantified. The number of transmigrated cells decreased during treatment compared to pre-treatment values: the lowest number of transmigrated cells was detected at T3, and, although transmigration was still lower at T12, there was a trend to a return to pre-treatment levels over time. The amount of MMP-9 also decreased during therapy, although we could not find an absolute correlation between transmigration and levels of MMP-9, nor between either parameter and the clinical course of patients.
| Original language | English |
|---|---|
| Pages (from-to) | 275-283 |
| Number of pages | 9 |
| Journal | Autoimmunity |
| Volume | 33 |
| Issue number | 4 |
| Publication status | Published - 2001 |
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Keywords
- β-IFN
- MMP-9
- Multiple sclerosis
- Transmigration
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
Cite this
Transmigration of PBMNCs from β-IFN-1b-treated MS patients : A one-year longitudinal study. / Gelati, M.; Corsini, E.; Dufour, A.; Zaffaroni, M.; Milanese, C.; La Mantia, L.; Massa, G.; Salmaggi, A.
In: Autoimmunity, Vol. 33, No. 4, 2001, p. 275-283.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Transmigration of PBMNCs from β-IFN-1b-treated MS patients
T2 - A one-year longitudinal study
AU - Gelati, M.
AU - Corsini, E.
AU - Dufour, A.
AU - Zaffaroni, M.
AU - Milanese, C.
AU - La Mantia, L.
AU - Massa, G.
AU - Salmaggi, A.
PY - 2001
Y1 - 2001
N2 - To evaluate the effects of in vivo β-IFN-1b treatment on transmigration of mononuclear cells, we monitored for one year in vitro mononuclear cells trafficking through HUVECs monolayers stratified over a collagen gel during β-IFN-1b treatment of 7 RR MS patients. The number of transmigrated cells was analysed before treatment (T0) and after 3 (T3), 6 (T6) and 12 months (T12); at the same time, levels of serum MMP-9 were quantified. The number of transmigrated cells decreased during treatment compared to pre-treatment values: the lowest number of transmigrated cells was detected at T3, and, although transmigration was still lower at T12, there was a trend to a return to pre-treatment levels over time. The amount of MMP-9 also decreased during therapy, although we could not find an absolute correlation between transmigration and levels of MMP-9, nor between either parameter and the clinical course of patients.
AB - To evaluate the effects of in vivo β-IFN-1b treatment on transmigration of mononuclear cells, we monitored for one year in vitro mononuclear cells trafficking through HUVECs monolayers stratified over a collagen gel during β-IFN-1b treatment of 7 RR MS patients. The number of transmigrated cells was analysed before treatment (T0) and after 3 (T3), 6 (T6) and 12 months (T12); at the same time, levels of serum MMP-9 were quantified. The number of transmigrated cells decreased during treatment compared to pre-treatment values: the lowest number of transmigrated cells was detected at T3, and, although transmigration was still lower at T12, there was a trend to a return to pre-treatment levels over time. The amount of MMP-9 also decreased during therapy, although we could not find an absolute correlation between transmigration and levels of MMP-9, nor between either parameter and the clinical course of patients.
KW - β-IFN
KW - MMP-9
KW - Multiple sclerosis
KW - Transmigration
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UR - http://www.scopus.com/inward/citedby.url?scp=0034847065&partnerID=8YFLogxK
M3 - Article
C2 - 11683402
AN - SCOPUS:0034847065
VL - 33
SP - 275
EP - 283
JO - Autoimmunity
JF - Autoimmunity
SN - 0891-6934
IS - 4
ER -