Transplantation of autologous endothelial cells induces angiogenesis

Valeri Chekanov, Massod Akhtar, Guennady Tchekanov, George Dangas, Muhammad Z. Shehzad, Fermin Tio, Milena Adamian, Antonio Colombo, Gary Roubin, Martin B. Leon, Jeffrey W. Moses, Nicholas N. Kipshidze

Research output: Contribution to journalArticle

Abstract

This study examined the feasibility and efficacy of autologous endothelial cell (EC) transplantation using a fibrin matrix in the ischemic myocardium of sheep. Four weeks after placing an ameroid constrictor in the circumflex artery of 12 adult sheep, four animals (EC group) were subjected to EC transplantation. In four others (saline [SAL] group) saline with added inactivated cells was injected and four animals served as controls. Eight weeks after treatment the animals were sacrificed to assess histology and ultrastructure. Eight weeks after injection, ventricular function was markedly improved in the EC transplant group, but had deteriorated in the SAL and control groups. Myocardial blood flow was also increased in the EC group. Histology and electron microscopy revealed extensive neovascularization after EC transplantation and improved myocardial appearance. Heterotopic transplantation of EC within a fibrin matrix enhances neovascularization, increases myocardial blood flow, and improves left ventricular function.

Original languageEnglish
Pages (from-to)496-499
Number of pages4
JournalPACE - Pacing and Clinical Electrophysiology
Volume26
Issue number1 II
Publication statusPublished - Jan 1 2003

Keywords

  • Angiogenesis
  • Endothelial cells
  • Fibrin
  • Transplantation

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Fingerprint Dive into the research topics of 'Transplantation of autologous endothelial cells induces angiogenesis'. Together they form a unique fingerprint.

  • Cite this

    Chekanov, V., Akhtar, M., Tchekanov, G., Dangas, G., Shehzad, M. Z., Tio, F., Adamian, M., Colombo, A., Roubin, G., Leon, M. B., Moses, J. W., & Kipshidze, N. N. (2003). Transplantation of autologous endothelial cells induces angiogenesis. PACE - Pacing and Clinical Electrophysiology, 26(1 II), 496-499.