Transplantation of chondrocytes seeded on a hyaluronan derivative (Hyaff®-11) into cartilage defects in rabbits

Brunella Grigolo, Livia Roseti, Mauro Fiorini, Milena Fini, Gianluca Giavaresi, Nicolò Nicoli Aldini, Roberto Giardino, Andrea Facchini

Research output: Contribution to journalArticle

Abstract

Different methods have been used to improve chondrocyte transplantation for the repair of articular cartilage defects. Several groups of biomaterials have been proposed as support for in vitro cell growth and for in vivo implantation. Here, we describe a new approach investigating the healing of rabbit cartilage by means of autologous chondrocytes seeded on a hyaluronan derivative referred to as Hyaff®-11. Full thickness defects were created bilaterally in the weight-bearing surface of the medial femoral condyle of both femora of New Zealand male rabbits. The wounds were then repaired using both chondrocytes seeded on the biomaterial and biomaterial alone. Controls were similarly treated but received either no treatment or implants of the delivery substance. Histologic samples from in and around the defect sites were examined 1, 3 and 6 months after surgery and were scored from 0 to 16. Statistically significant differences in the quality of the regenerated tissue were found between the grafts carried out with biomaterial carrying chondrocyte cells compared to the biomaterial alone or controls. This study demonstrates the efficacy of this hyaluronan-based scaffold for autologous chondrocytes transplantation.

Original languageEnglish
Pages (from-to)2417-2424
Number of pages8
JournalBiomaterials
Volume22
Issue number17
DOIs
Publication statusPublished - 2001

Fingerprint

Cartilage
Biocompatible Materials
Hyaluronic Acid
Chondrocytes
Biomaterials
Transplantation
Rabbits
Derivatives
Defects
Bearings (structural)
Autologous Transplantation
Weight-Bearing
Cell growth
Articular Cartilage
Thigh
Grafts
Scaffolds
Femur
Surgery
Repair

Keywords

  • Articular cartilage
  • Chondrocytes
  • Hyaff-11
  • Transplantation

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Transplantation of chondrocytes seeded on a hyaluronan derivative (Hyaff®-11) into cartilage defects in rabbits. / Grigolo, Brunella; Roseti, Livia; Fiorini, Mauro; Fini, Milena; Giavaresi, Gianluca; Nicoli Aldini, Nicolò; Giardino, Roberto; Facchini, Andrea.

In: Biomaterials, Vol. 22, No. 17, 2001, p. 2417-2424.

Research output: Contribution to journalArticle

@article{0f45a243076345b39b34caf2ea85b468,
title = "Transplantation of chondrocytes seeded on a hyaluronan derivative (Hyaff{\circledR}-11) into cartilage defects in rabbits",
abstract = "Different methods have been used to improve chondrocyte transplantation for the repair of articular cartilage defects. Several groups of biomaterials have been proposed as support for in vitro cell growth and for in vivo implantation. Here, we describe a new approach investigating the healing of rabbit cartilage by means of autologous chondrocytes seeded on a hyaluronan derivative referred to as Hyaff{\circledR}-11. Full thickness defects were created bilaterally in the weight-bearing surface of the medial femoral condyle of both femora of New Zealand male rabbits. The wounds were then repaired using both chondrocytes seeded on the biomaterial and biomaterial alone. Controls were similarly treated but received either no treatment or implants of the delivery substance. Histologic samples from in and around the defect sites were examined 1, 3 and 6 months after surgery and were scored from 0 to 16. Statistically significant differences in the quality of the regenerated tissue were found between the grafts carried out with biomaterial carrying chondrocyte cells compared to the biomaterial alone or controls. This study demonstrates the efficacy of this hyaluronan-based scaffold for autologous chondrocytes transplantation.",
keywords = "Articular cartilage, Chondrocytes, Hyaff-11, Transplantation",
author = "Brunella Grigolo and Livia Roseti and Mauro Fiorini and Milena Fini and Gianluca Giavaresi and {Nicoli Aldini}, Nicol{\`o} and Roberto Giardino and Andrea Facchini",
year = "2001",
doi = "10.1016/S0142-9612(00)00429-4",
language = "English",
volume = "22",
pages = "2417--2424",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier BV",
number = "17",

}

TY - JOUR

T1 - Transplantation of chondrocytes seeded on a hyaluronan derivative (Hyaff®-11) into cartilage defects in rabbits

AU - Grigolo, Brunella

AU - Roseti, Livia

AU - Fiorini, Mauro

AU - Fini, Milena

AU - Giavaresi, Gianluca

AU - Nicoli Aldini, Nicolò

AU - Giardino, Roberto

AU - Facchini, Andrea

PY - 2001

Y1 - 2001

N2 - Different methods have been used to improve chondrocyte transplantation for the repair of articular cartilage defects. Several groups of biomaterials have been proposed as support for in vitro cell growth and for in vivo implantation. Here, we describe a new approach investigating the healing of rabbit cartilage by means of autologous chondrocytes seeded on a hyaluronan derivative referred to as Hyaff®-11. Full thickness defects were created bilaterally in the weight-bearing surface of the medial femoral condyle of both femora of New Zealand male rabbits. The wounds were then repaired using both chondrocytes seeded on the biomaterial and biomaterial alone. Controls were similarly treated but received either no treatment or implants of the delivery substance. Histologic samples from in and around the defect sites were examined 1, 3 and 6 months after surgery and were scored from 0 to 16. Statistically significant differences in the quality of the regenerated tissue were found between the grafts carried out with biomaterial carrying chondrocyte cells compared to the biomaterial alone or controls. This study demonstrates the efficacy of this hyaluronan-based scaffold for autologous chondrocytes transplantation.

AB - Different methods have been used to improve chondrocyte transplantation for the repair of articular cartilage defects. Several groups of biomaterials have been proposed as support for in vitro cell growth and for in vivo implantation. Here, we describe a new approach investigating the healing of rabbit cartilage by means of autologous chondrocytes seeded on a hyaluronan derivative referred to as Hyaff®-11. Full thickness defects were created bilaterally in the weight-bearing surface of the medial femoral condyle of both femora of New Zealand male rabbits. The wounds were then repaired using both chondrocytes seeded on the biomaterial and biomaterial alone. Controls were similarly treated but received either no treatment or implants of the delivery substance. Histologic samples from in and around the defect sites were examined 1, 3 and 6 months after surgery and were scored from 0 to 16. Statistically significant differences in the quality of the regenerated tissue were found between the grafts carried out with biomaterial carrying chondrocyte cells compared to the biomaterial alone or controls. This study demonstrates the efficacy of this hyaluronan-based scaffold for autologous chondrocytes transplantation.

KW - Articular cartilage

KW - Chondrocytes

KW - Hyaff-11

KW - Transplantation

UR - http://www.scopus.com/inward/record.url?scp=0034918532&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034918532&partnerID=8YFLogxK

U2 - 10.1016/S0142-9612(00)00429-4

DO - 10.1016/S0142-9612(00)00429-4

M3 - Article

C2 - 11511039

AN - SCOPUS:0034918532

VL - 22

SP - 2417

EP - 2424

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 17

ER -