Transplanted human adipose tissue-derived stem cells engraft and induce regeneration in mice olfactory neuroepithelium in response to dichlobenil subministration

Valeria Franceschini, Simone Bettini, Simone Pifferi, Anna Menini, Gabriele Siciliano, Emanuela Ognio, Anna Teresa Brini, Enrico Di Oto, Roberto P. Revoltella

Research output: Contribution to journalArticlepeer-review

Abstract

We used immunodeficient mice, whose dorsomedial olfactory region was permanently damaged by dichlobenil inoculation, to test the neuroregenerative properties of transplanted human adipose tissue-derived stem cells after 30 and 60 days. Analysis of polymerase chain reaction bands revealed that stem cells preferentially engrafted in the lesioned olfactory epithelium compared with undamaged mucosa of untreated transplanted mice. Although basal cell proliferation in untransplanted lesioned mice did not give rise to neuronal cells in the olfactory mucosa, we observed clusters of differentiating olfactory cells in transplanted mice. After 30 days, and even more at 60 days, epithelial thickness was partially recovered to normal values, as also the immunohistochemical properties. Functional reactivity to odorant stimulation was also confirmed through electroolfactogram recording in the dorsomedial epithelium. Furthermore, we demonstrated that engrafted stem cells fused with mouse cells in the olfactory organ, even if heterokaryons detected were too rare to hypothesize they directly repopulated the lesioned epithelium. The data reported prove that the migrating transplanted stem cells were able to induce a neuroregenerative process in a specific lesioned sensory area, enforcing the perspective that they could become an available tool for stem cell therapy.

Original languageEnglish
Article numberbju035
JournalChemical Senses
Volume39
Issue number7
DOIs
Publication statusPublished - 2014

Keywords

  • Herbicide
  • Nod-scid mice
  • Olfactory mucosa
  • Stem cell transplantation
  • Tissue recovery

ASJC Scopus subject areas

  • Behavioral Neuroscience
  • Physiology
  • Physiology (medical)
  • Sensory Systems
  • Medicine(all)

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