Transport of soluble proteins through the Golgi occurs by diffusion via continuities across cisternae

Galina V. Beznoussenko; Seetharaman Parashuraman; Riccardo Rizzo; Roman Polishchuk; Oliviano Martella; Daniele Di Giandomenico; Aurora Fusella; Alexander Spaar; Michele Sallese; Maria G razia Capestrano; Margit Pavelka; Matthijn R. Vos; Yuri G M Rikers; Volkhard Helms; Alexandre A. Mironov; Alberto Luini

doi:10.7554/eLife.02009

Transport of soluble proteins through the Golgi occurs by diffusion via continuities across cisternae

Galina V. Beznoussenko, Seetharaman Parashuraman, Riccardo Rizzo, Roman Polishchuk, Oliviano Martella, Daniele Di Giandomenico, Aurora Fusella, Alexander Spaar, Michele Sallese, Maria G razia Capestrano, Margit Pavelka, Matthijn R. Vos, Yuri G M Rikers, Volkhard Helms, Alexandre A. Mironov, Alberto Luini

IRCCS SDN

Research output: Contribution to journal › Article › peer-review

Abstract

The mechanism of transport through the Golgi complex is not completely understood, insofar as no single transport mechanism appears to account for all of the observations. Here, we compare the transport of soluble secretory proteins (albumin and α1-antitrypsin) with that of supramolecular cargoes (e.g., procollagen) that are proposed to traverse the Golgi by compartment progression-maturation. We show that these soluble proteins traverse the Golgi much faster than procollagen while moving through the same stack. Moreover, we present kinetic and morphological observations that indicate that albumin transport occurs by diffusion via intercisternal continuities. These data provide evidence for a transport mechanism that applies to a major class of secretory proteins and indicate the co-existence of multiple intra-Golgi trafficking modes.

Original language	English
Journal	eLife
Volume	3
DOIs	https://doi.org/10.7554/eLife.02009
Publication status	Published - 2014

Keywords

albumin
cell biology
golgi complex
human
intracellular trafficking
membrane tubules
soluble cargo proteins

ASJC Scopus subject areas

Neuroscience(all)
Medicine(all)
Immunology and Microbiology(all)
Biochemistry, Genetics and Molecular Biology(all)

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10.7554/eLife.02009

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Beznoussenko, G. V., Parashuraman, S., Rizzo, R., Polishchuk, R., Martella, O., Di Giandomenico, D., Fusella, A., Spaar, A., Sallese, M., Capestrano, M. G. R., Pavelka, M., Vos, M. R., Rikers, Y. G. M., Helms, V., Mironov, A. A., & Luini, A. (2014). Transport of soluble proteins through the Golgi occurs by diffusion via continuities across cisternae. eLife, 3. https://doi.org/10.7554/eLife.02009

Transport of soluble proteins through the Golgi occurs by diffusion via continuities across cisternae. / Beznoussenko, Galina V.; Parashuraman, Seetharaman; Rizzo, Riccardo; Polishchuk, Roman; Martella, Oliviano; Di Giandomenico, Daniele; Fusella, Aurora; Spaar, Alexander; Sallese, Michele; Capestrano, Maria G razia; Pavelka, Margit; Vos, Matthijn R.; Rikers, Yuri G M; Helms, Volkhard; Mironov, Alexandre A.; Luini, Alberto.

In: eLife, Vol. 3, 2014.

Research output: Contribution to journal › Article › peer-review

Beznoussenko, GV, Parashuraman, S, Rizzo, R, Polishchuk, R, Martella, O, Di Giandomenico, D, Fusella, A, Spaar, A, Sallese, M, Capestrano, MGR, Pavelka, M, Vos, MR, Rikers, YGM, Helms, V, Mironov, AA & Luini, A 2014, 'Transport of soluble proteins through the Golgi occurs by diffusion via continuities across cisternae', eLife, vol. 3. https://doi.org/10.7554/eLife.02009

Beznoussenko, Galina V. ; Parashuraman, Seetharaman ; Rizzo, Riccardo ; Polishchuk, Roman ; Martella, Oliviano ; Di Giandomenico, Daniele ; Fusella, Aurora ; Spaar, Alexander ; Sallese, Michele ; Capestrano, Maria G razia ; Pavelka, Margit ; Vos, Matthijn R. ; Rikers, Yuri G M ; Helms, Volkhard ; Mironov, Alexandre A. ; Luini, Alberto. / Transport of soluble proteins through the Golgi occurs by diffusion via continuities across cisternae. In: eLife. 2014 ; Vol. 3.

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abstract = "The mechanism of transport through the Golgi complex is not completely understood, insofar as no single transport mechanism appears to account for all of the observations. Here, we compare the transport of soluble secretory proteins (albumin and α1-antitrypsin) with that of supramolecular cargoes (e.g., procollagen) that are proposed to traverse the Golgi by compartment progression-maturation. We show that these soluble proteins traverse the Golgi much faster than procollagen while moving through the same stack. Moreover, we present kinetic and morphological observations that indicate that albumin transport occurs by diffusion via intercisternal continuities. These data provide evidence for a transport mechanism that applies to a major class of secretory proteins and indicate the co-existence of multiple intra-Golgi trafficking modes. ",

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AU - Polishchuk, Roman

AU - Martella, Oliviano

AU - Di Giandomenico, Daniele

AU - Fusella, Aurora

AU - Spaar, Alexander

AU - Sallese, Michele

AU - Capestrano, Maria G razia

AU - Pavelka, Margit

AU - Vos, Matthijn R.

AU - Rikers, Yuri G M

AU - Helms, Volkhard

AU - Mironov, Alexandre A.

AU - Luini, Alberto

PY - 2014

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KW - albumin

KW - cell biology

KW - golgi complex

KW - human

KW - intracellular trafficking

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Transport of soluble proteins through the Golgi occurs by diffusion via continuities across cisternae

Abstract

Keywords

ASJC Scopus subject areas

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