Transport of soluble proteins through the Golgi occurs by diffusion via continuities across cisternae

Galina V. Beznoussenko, Seetharaman Parashuraman, Riccardo Rizzo, Roman Polishchuk, Oliviano Martella, Daniele Di Giandomenico, Aurora Fusella, Alexander Spaar, Michele Sallese, Maria G razia Capestrano, Margit Pavelka, Matthijn R. Vos, Yuri G M Rikers, Volkhard Helms, Alexandre A. Mironov, Alberto Luini

Research output: Contribution to journalArticlepeer-review

Abstract

The mechanism of transport through the Golgi complex is not completely understood, insofar as no single transport mechanism appears to account for all of the observations. Here, we compare the transport of soluble secretory proteins (albumin and α1-antitrypsin) with that of supramolecular cargoes (e.g., procollagen) that are proposed to traverse the Golgi by compartment progression-maturation. We show that these soluble proteins traverse the Golgi much faster than procollagen while moving through the same stack. Moreover, we present kinetic and morphological observations that indicate that albumin transport occurs by diffusion via intercisternal continuities. These data provide evidence for a transport mechanism that applies to a major class of secretory proteins and indicate the co-existence of multiple intra-Golgi trafficking modes.

Original languageEnglish
JournaleLife
Volume3
DOIs
Publication statusPublished - 2014

Keywords

  • albumin
  • cell biology
  • golgi complex
  • human
  • intracellular trafficking
  • membrane tubules
  • soluble cargo proteins

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'Transport of soluble proteins through the Golgi occurs by diffusion via continuities across cisternae'. Together they form a unique fingerprint.

Cite this