Treadmill exercise-induced release of endothelin-1 in patients with peripheral arterial occlusive disease at Fontaine stage IIb

R. A. Mangiafico, L. S. Malatino, R. S. Spada, M. Santonocito, R. Messina, S. Dell'arte, T. Attinà

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Abstract

Background. Endothelin-1 (ET-1) is an endothelial vasoconstrictor mitogenic peptide which is thought to be a marker of endothelial damage and a potential participant in the pathophysiological processes of the development of atherosclerotic lesions and disease states associated with vasoconstriction and vasospasm. Methods. To investigate the endothelin-1 release in response to dynamic exercise in patients with peripheral arterial occlusive disease (PAOD), plasma concentrations were determined by radioimmunoassay in 16 patients (14 men, 2 women, mean age 56.2 ± 8.1 years) with peripheral arterial occlusive disease at Fontaine stage IIb and in 10 control subjects (8 men, 2 women, mean age 58.1 ± 7.2 years) in normal health during treadmill testing (slope 5%, speed 3 km/hr). Blood samples were collected at rest from an antecubital vein, at the onset of claudication pain, and 10 minutes after exercise. Results. Mean plasma endothelin-1 concentrations during the stress test increased significantly in the patients with arterial disease, rising from basal values of 4.4 ± 0.6 pmol/L to values of 8.9 ± 0.7 pmol/L at the end of the test (p <0.0001), whereas it did not change significantly in control subjects (rising from 2.6 ± 0.4 pmol/L to 2.7 ± 0.5 pmol/L). Further, plasma endothelin-1 in the patients with arterial disease was at all times higher than in the control subjects (p <0.0001). Conclusions. In conclusion, this study shows that in patients with peripheral arterial occlusive disease, plasma endothelin-1 increases after treadmill exercise performed until claudication pain supervenes. Raised endothelin-1 could be a marker of ischaemic acute endothelial damage and/or could contribute to increase the vascular resistance in ischaemic limbs of these patients during dynamic exercise by promoting arterial/arteriolar vasoconstriction or vasospasm.

Original languageEnglish
Pages (from-to)14-17
Number of pages4
JournalInternational Angiology
Volume19
Issue number1
Publication statusPublished - 2000

Keywords

  • Arterial occlusive diseases
  • Endothelin-1
  • Exercise test
  • Peripheral vascular diseases

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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    Mangiafico, R. A., Malatino, L. S., Spada, R. S., Santonocito, M., Messina, R., Dell'arte, S., & Attinà, T. (2000). Treadmill exercise-induced release of endothelin-1 in patients with peripheral arterial occlusive disease at Fontaine stage IIb. International Angiology, 19(1), 14-17.