Treatment of adjuvant arthritis with granulocyte-colony stimulating factor and peptide derived from heat shock protein 65

Andrea Brendolan, Masanori Higuchi, Richard Sibley, Samuel Strober

Research output: Contribution to journalArticlepeer-review

Abstract

Adjuvant arthritis in Lewis rats is induced by the subcutaneous injection of Mycobacterium tuberculosis in mineral oil, and the predominant T cell immune reactivity is against the heat shock protein 65 derived peptide 176-190. We treated Lewis rats with human recombinant G-CSF followed by (i.v) administration of peptide 176-190 after induction of adjuvant arthritis (AA), and observed decreased disease severity, joint destruction, new bone formation and joint ankylosis. Treatment with G-CSF alone was also effective, but to a lesser extent. In addition, we found that splenocytes from rats treated with G-CSF had reduced antigen presenting capacity compared with splenocytes from vehicle treated rats. Primed lymph node cells from G-CSF plus peptide treated rats showed a marked reduction in proliferation and secretion of IFN-γ after stimulation with the heat shock protein peptide in vitro as compared to controls.

Original languageEnglish
Pages (from-to)6-14
Number of pages9
JournalCellular Immunology
Volume221
Issue number1
DOIs
Publication statusPublished - Jan 2003

Keywords

  • Adjuvant arthritis
  • Granulocyte-colony stimulating factor
  • Heat shock protein
  • Immunoregulation

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

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