Abstract
Adjuvant arthritis in Lewis rats is induced by the subcutaneous injection of Mycobacterium tuberculosis in mineral oil, and the predominant T cell immune reactivity is against the heat shock protein 65 derived peptide 176-190. We treated Lewis rats with human recombinant G-CSF followed by (i.v) administration of peptide 176-190 after induction of adjuvant arthritis (AA), and observed decreased disease severity, joint destruction, new bone formation and joint ankylosis. Treatment with G-CSF alone was also effective, but to a lesser extent. In addition, we found that splenocytes from rats treated with G-CSF had reduced antigen presenting capacity compared with splenocytes from vehicle treated rats. Primed lymph node cells from G-CSF plus peptide treated rats showed a marked reduction in proliferation and secretion of IFN-γ after stimulation with the heat shock protein peptide in vitro as compared to controls.
Original language | English |
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Pages (from-to) | 6-14 |
Number of pages | 9 |
Journal | Cellular Immunology |
Volume | 221 |
Issue number | 1 |
DOIs | |
Publication status | Published - Jan 2003 |
Keywords
- Adjuvant arthritis
- Granulocyte-colony stimulating factor
- Heat shock protein
- Immunoregulation
ASJC Scopus subject areas
- Cell Biology
- Immunology