TY - JOUR
T1 - Treatment of chondral defects of the knee with one step matrix-assisted technique enhanced by autologous concentrated bone marrow
T2 - In vitro characterisation of mesenchymal stem cells from iliac crest and subchondral bone
AU - De Girolamo, Laura
AU - Bertolini, Giulia
AU - Cervellin, Matteo
AU - Sozzi, Gabriella
AU - Volpi, Piero
PY - 2010/11
Y1 - 2010/11
N2 - Cartilage repair is still an unsolved problem. In the last years many cell-based treatments have been proposed, in order to obtain good regeneration of cartilage defects. The Autologous Matrix-Induced Chondrogenesis technique (AMIC
®) combines the micro-fracture procedure with the use of a specific biological membrane. The phenotypic feature of bone marrow cell population, harvested from iliac crest and knee subchondral bone of patients treated with the AMIC
® technique, enhanced by autologous concentrated bone marrow, was analysed to evaluate potential variations of the cell population. Samples of eleven patients, with isolated chondral lesions grade III or IV were treated with the AMIC
® technique, enhanced by the use of autologous concentrated bone marrow. A small fraction of bone marrow samples, both from iliac crest and from the created micro-fractures, was analysed by FACS analysis and then cultured to verify their proliferative and differentiation potential. An average of 0.04% of concentrated bone marrow cells harvested from the iliac crest, presented mesenchymal stem cell phenotype (CD34
-/CD45
low/CD271
high), whereas just 0.02% of these cells were identified from the samples harvested during the creation of micro-fractures at the knee. After two passages in culture, cells expressed a peculiar profile for MSC. Only MSC from bone marrow could be long-term propagated and were able to efficiently differentiate in the cultures. Although the AMIC
® approach has many advantages, the surgical technique in the application of the microfracture technique remains essential and affects the final result.
AB - Cartilage repair is still an unsolved problem. In the last years many cell-based treatments have been proposed, in order to obtain good regeneration of cartilage defects. The Autologous Matrix-Induced Chondrogenesis technique (AMIC
®) combines the micro-fracture procedure with the use of a specific biological membrane. The phenotypic feature of bone marrow cell population, harvested from iliac crest and knee subchondral bone of patients treated with the AMIC
® technique, enhanced by autologous concentrated bone marrow, was analysed to evaluate potential variations of the cell population. Samples of eleven patients, with isolated chondral lesions grade III or IV were treated with the AMIC
® technique, enhanced by the use of autologous concentrated bone marrow. A small fraction of bone marrow samples, both from iliac crest and from the created micro-fractures, was analysed by FACS analysis and then cultured to verify their proliferative and differentiation potential. An average of 0.04% of concentrated bone marrow cells harvested from the iliac crest, presented mesenchymal stem cell phenotype (CD34
-/CD45
low/CD271
high), whereas just 0.02% of these cells were identified from the samples harvested during the creation of micro-fractures at the knee. After two passages in culture, cells expressed a peculiar profile for MSC. Only MSC from bone marrow could be long-term propagated and were able to efficiently differentiate in the cultures. Although the AMIC
® approach has many advantages, the surgical technique in the application of the microfracture technique remains essential and affects the final result.
KW - Bone marrow
KW - Chondral lesion
KW - Chondrogenic potential
KW - Matrix-assisted technique
KW - Mesenchymal stem cells
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U2 - 10.1016/j.injury.2010.09.027
DO - 10.1016/j.injury.2010.09.027
M3 - Article
C2 - 20934693
AN - SCOPUS:78049273159
VL - 41
SP - 1172
EP - 1177
JO - Injury
JF - Injury
SN - 0020-1383
IS - 11
ER -