Treatment of Periodontal Ligament Stem Cells with MOR and CBD Promotes Cell Survival and Neuronal Differentiation via the PI3K/Akt/mTOR Pathway

Veronica Lanza Cariccio, Domenico Scionti, Antonio Raffa, Renato Iori, Federica Pollastro, Francesca Diomede, Placido Bramanti, Oriana Trubiani, Emanuela Mazzon

Research output: Contribution to journalArticlepeer-review

Abstract

Periodontal ligament mesenchymal stem cells (hPDLSCs), as well as all mesenchymal stem cells, show self-renewal, clonogenicity, and multi-tissue differentiation proprieties and can represent a valid support for regenerative medicine. We treated hPDLSCs with a combination of Moringin (MOR) and Cannabidiol (CBD), in order to understand if treatment could improve their survival and their in vitro differentiation capacity. Stem cells survival is fundamental to achieve a successful therapy outcome in the re-implanted tissue of patients. Through NGS transcriptome analysis, we found that combined treatment increased hPDLSCs survival, by inhibition of apoptosis as demonstrated by enhanced expression of anti-apoptotic genes and reduction of pro-apoptotic ones. Moreover, we investigated the possible involvement of PI3K/Akt/mTOR pathway, emphasizing a differential gene expression between treated and untreated cells. Furthermore, hPDLSCs were cultured for 48 h in the presence or absence of CBD and MOR and, after confirming the cellular viability through MTT (3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazoliumbromide) assay, we examined the presence of neuronal markers, through immunofluorescence analysis. We found an increased expression of Nestin and GAP43 (growth associated protein 43) in treated cells. In conclusion, hPDLSCs treated with Moringin and Cannabidiol showed an improved survival capacity and neuronal differentiation potential.

Original languageEnglish
JournalInternational Journal of Molecular Sciences
Volume19
Issue number8
DOIs
Publication statusPublished - Aug 9 2018

Keywords

  • Apoptosis/drug effects
  • Cannabidiol/pharmacology
  • Cell Differentiation/drug effects
  • Cell Proliferation/drug effects
  • Cell Self Renewal/drug effects
  • Cell Survival/drug effects
  • GAP-43 Protein/genetics
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental/drug effects
  • Humans
  • Isothiocyanates/pharmacology
  • Mesenchymal Stem Cells/cytology
  • Nestin/genetics
  • Neurons/drug effects
  • Osteogenesis/drug effects
  • Periodontal Ligament/cytology

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