Treatment with 6-hydroxydopamine in planaria (Dugesia gonocephala s.l.): Morphological and behavioral study

Brunella Caronti, Vito Margotta, Angelo Merante, Francesco E. Pontieri, Guido Palladini

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Morpho-functional and behavioral effects of exposure to 6-hydroxydopamine (OHDA)-HCl (24 μg/ml per day for 24 h and 7 days) were studied in planarias (Dugesia gonocephala s.l.). Exposure to 6-OHDA-HCl for 24 h produced hypokinesia of the specimens. These behavioral changes were more pronounced, leading to complete immobility, after 7 days of exposure to the neurotoxin. Moreover, specimens exposed to 6-OHDA-HCl for 24 h and 7 days failed to show any behavioral response to nomifensine, thus furnishing evidence of the damage of presynaptic dopamine terminals. Exposure to 6-OHDA-HCl for 24 h significantly reduced cathecolamine content in neuropil region, as demonstrated by histochemistry, and electron-dense presynaptic vesicles, as observed on electron microscopy examination. All these alterations were significantly more pronounced and were accompanied by swelling and strong increase of electron-density in cytoplasm of numerous neurons after exposure to the neurotoxin for 7 days. This appears to be the first demonstration of the neurotoxic effects of 6-OHDA-HCl in flatworms. Copyright (C) 1999 Elsevier Science Inc.

Original languageEnglish
Pages (from-to)201-207
Number of pages7
JournalComparative Biochemistry and Physiology - C Pharmacology Toxicology and Endocrinology
Volume123
Issue number3
DOIs
Publication statusPublished - Jul 1999

Fingerprint

Planarians
Oxidopamine
Neurotoxins
Platyhelminths
Nomifensine
Electrons
Hypokinesia
Neuropil
Presynaptic Terminals
Dopamine
Electron Microscopy
Cytoplasm
Neurons

Keywords

  • Catecholamines
  • Degeneration
  • Nervous system
  • Turbellaria

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

Cite this

Treatment with 6-hydroxydopamine in planaria (Dugesia gonocephala s.l.) : Morphological and behavioral study. / Caronti, Brunella; Margotta, Vito; Merante, Angelo; Pontieri, Francesco E.; Palladini, Guido.

In: Comparative Biochemistry and Physiology - C Pharmacology Toxicology and Endocrinology, Vol. 123, No. 3, 07.1999, p. 201-207.

Research output: Contribution to journalArticle

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