Treatment with Angiotensin-(1-7) reduces inflammation in carotid atherosclerotic plaques

Rodrigo A. Fraga-Silva, Silvia Q. Savergnini, Fabrizio Montecucco, Alessio Nencioni, Irene Caffa, Debora Soncini, Fabiana P. Costa-Fraga, Frederico B. De Sousa, Ruben D. Sinisterra, Luciano A S Capettini, Sébastien Lenglet, Katia Galan, Graziano Pelli, Maria Bertolotto, Aldo Pende, Giovanni Spinella, Bianca Pane, Franco Dallegri, Domenico Palombo, François MachNikolaos Stergiopulos, Robson A S Santos, Rafaela F. da Silva

Research output: Contribution to journalArticlepeer-review

Abstract

Angiotensin (Ang)-(1-7), acting through the receptor Mas, has atheroprotective effects; however, its role on plaque vulnerability has been poorly studied. Here, we investigated the expression of the renin-angiotensin system (RAS) components in stable and unstable human carotid plaques. In addition, we evaluated the effects of the chronic treatment with an oral formulation of Ang-(1-7) in a mouse model of shear stress-determined carotid atherosclerotic plaque. Upstream and downstream regions of internal carotid plaques were obtained from a recently published cohort of patients asymptomatic or symptomatic for ischaemic stroke. Angiotensinogen and renin genes were strongly expressed in the entire cohort, indicating an intense intraplaque modulation of the RAS. Intraplaque expression of the Mas receptor mRNA was increased in the downstream portion of asymptomatic patients as compared to corresponding region in symptomatic patients. Conversely, AT1 receptor gene expression was not modified between asymptomatic and symptomatic patients. Treatment with Ang-(1-7) in ApoE-/- mice was associated with increased intraplaque collagen content in the aortic root and low shear stress-induced carotid plaques, and a decreased MMP-9 content and neutrophil and macrophage infiltration. These beneficial effects were not observed in the oscillatory shear stress-induced plaque. In vitro incubation with Ang-(1-7) did not affect ICAM-1 expression and apoptosis on cultured endothelial cells. In conclusion, Mas receptor is up regulated in the downstream portions of human stable carotid plaques as compared to unstable lesions. Treatment with the oral formulation of Ang-(1-7) enhances a more stable phenotype in atherosclerotic plaques, depending on the local pattern of shear stress forces.

Original languageEnglish
Pages (from-to)736-747
Number of pages12
JournalThrombosis and Haemostasis
Volume111
Issue number4
DOIs
Publication statusPublished - Feb 6 2014

Keywords

  • Angiotensin
  • Atherosclerosis
  • Inflammation
  • Plaque stability

ASJC Scopus subject areas

  • Hematology
  • Medicine(all)

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