Trends in production of extended-spectrum β-lactamases among enterobacteria of medical interest: Report of the second Italian nationwide survey

Francesco Luzzaro, Marilina Mezzatesta, Claudia Mugnaioli, Mariagrazia Perilli, Stefania Stefani, Gianfranco Amicosante, Gian Maria Rossolini, Antonio Toniolo

Research output: Contribution to journalArticle

Abstract

Results of a 2003 survey carried out in Italy to evaluate the prevalence of extended-spectrum β-lactamase (ESBL)-producing enterobacteria are presented. Eleven Italian Microbiology Laboratories investigated 9,076 consecutive nonreplicate isolates (inpatients, 6,850; outpatients, 2,226). ESBL screening was performed by MIC data analysis. Confirmation was obtained using the double-disk synergy test and the combination disk test based on CLSI methodology. ESBL determinants were investigated by colony blot hybridization and confirmed by sequencing. Results were compared to those of the 1999 Italian survey (8,015 isolates). The prevalence of ESBL producers was 7.4% among isolates from inpatients (in 1999, 6.3%) and 3.5% among outpatients (no data were available for 1999). Among hospitalized patients, the most prevalent ESBL-positive species was Escherichia coli (Klebsiella pneumoniae in 1999). Proteus mirabilis was the most prevalent ESBL-positive species among outpatients. In both groups, most ESBL-positive pathogens were obtained from urinary tract infections. TEM-type ESBLs were the most prevalent enzymes (45.4%). Non-TEM, non-SHV determinants emerged: CTX-M-type in E. coli and K. pneumoniae, and PER-type in P. mirabilis, Providencia spp., and E. coli. With the exception of 3/163 P. mirabilis isolates and 1/44 Providencia stuartii isolate (all of which were intermediate for imipenem), carbapenems were active against all ESBL-positive enterobacteria. Susceptibility to other drugs was as follows: 84.7% for amikacin, 84.4% for piperacillin-tazobactam, 48.0% for gentamicin, and 32.8% for ciprofloxacin. Carbapenems appear to be the drug of choice. Amikacin and β-lactam/β-lactamase inhibitor combinations represent an alternative in non-life-threatening infections. The appearance of ESBL-positive enterobacteria in the community makes it mandatory that family physicians learn how to treat these pathogens.

Original languageEnglish
Pages (from-to)1659-1664
Number of pages6
JournalJournal of Clinical Microbiology
Volume44
Issue number5
DOIs
Publication statusPublished - May 2006

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Proteus mirabilis
Enterobacteriaceae
Providencia
Carbapenems
Outpatients
Amikacin
Klebsiella pneumoniae
Escherichia coli
Inpatients
Lactams
Imipenem
Family Physicians
Ciprofloxacin
Microbiology
Gentamicins
Urinary Tract Infections
Pharmaceutical Preparations
Italy
Enzymes
Infection

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

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Trends in production of extended-spectrum β-lactamases among enterobacteria of medical interest : Report of the second Italian nationwide survey. / Luzzaro, Francesco; Mezzatesta, Marilina; Mugnaioli, Claudia; Perilli, Mariagrazia; Stefani, Stefania; Amicosante, Gianfranco; Rossolini, Gian Maria; Toniolo, Antonio.

In: Journal of Clinical Microbiology, Vol. 44, No. 5, 05.2006, p. 1659-1664.

Research output: Contribution to journalArticle

Luzzaro, F, Mezzatesta, M, Mugnaioli, C, Perilli, M, Stefani, S, Amicosante, G, Rossolini, GM & Toniolo, A 2006, 'Trends in production of extended-spectrum β-lactamases among enterobacteria of medical interest: Report of the second Italian nationwide survey', Journal of Clinical Microbiology, vol. 44, no. 5, pp. 1659-1664. https://doi.org/10.1128/JCM.44.5.1659-1664.2006
Luzzaro, Francesco ; Mezzatesta, Marilina ; Mugnaioli, Claudia ; Perilli, Mariagrazia ; Stefani, Stefania ; Amicosante, Gianfranco ; Rossolini, Gian Maria ; Toniolo, Antonio. / Trends in production of extended-spectrum β-lactamases among enterobacteria of medical interest : Report of the second Italian nationwide survey. In: Journal of Clinical Microbiology. 2006 ; Vol. 44, No. 5. pp. 1659-1664.
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