TRF2 and Apollo Cooperate with Topoisomerase 2α to Protect Human Telomeres from Replicative Damage

Jing Ye, Christelle Lenain, Serge Bauwens, Angela Rizzo, Adelaïde Saint-Léger, Anaïs Poulet, Delphine Benarroch, Frédérique Magdinier, Julia Morere, Simon Amiard, Els Verhoeyen, Sébastien Britton, Patrick Calsou, Bernard Salles, Anna Bizard, Marc Nadal, Erica Salvati, Laure Sabatier, Yunlin Wu, Annamaria BiroccioArturo Londoño-Vallejo, Marie Josèphe Giraud-Panis, Eric Gilson

Research output: Contribution to journalArticle

Abstract

Human telomeres are protected from DNA damage by a nucleoprotein complex that includes the repeat-binding factor TRF2. Here, we report that TRF2 regulates the 5' exonuclease activity of its binding partner, Apollo, a member of the metallo-β-lactamase family that is required for telomere integrity during S phase. TRF2 and Apollo also suppress damage to engineered interstitial telomere repeat tracts that were inserted far away from chromosome ends. Genetic data indicate that DNA topoisomerase 2α acts in the same pathway of telomere protection as TRF2 and Apollo. Moreover, TRF2, which binds preferentially to positively supercoiled DNA substrates, together with Apollo, negatively regulates the amount of TOP1, TOP2α, and TOP2β at telomeres. Our data are consistent with a model in which TRF2 and Apollo relieve topological stress during telomere replication. Our work also suggests that cellular senescence may be caused by topological problems that occur during the replication of the inner portion of telomeres.

Original languageEnglish
Pages (from-to)230-242
Number of pages13
JournalCell
Volume142
Issue number2
DOIs
Publication statusPublished - Jul 2010

Fingerprint

Phosphodiesterase I
Superhelical DNA
Type I DNA Topoisomerase
Nucleoproteins
Telomere
Chromosomes
DNA
Substrates
Cell Aging
S Phase
DNA Damage

Keywords

  • DNA

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Ye, J., Lenain, C., Bauwens, S., Rizzo, A., Saint-Léger, A., Poulet, A., ... Gilson, E. (2010). TRF2 and Apollo Cooperate with Topoisomerase 2α to Protect Human Telomeres from Replicative Damage. Cell, 142(2), 230-242. https://doi.org/10.1016/j.cell.2010.05.032

TRF2 and Apollo Cooperate with Topoisomerase 2α to Protect Human Telomeres from Replicative Damage. / Ye, Jing; Lenain, Christelle; Bauwens, Serge; Rizzo, Angela; Saint-Léger, Adelaïde; Poulet, Anaïs; Benarroch, Delphine; Magdinier, Frédérique; Morere, Julia; Amiard, Simon; Verhoeyen, Els; Britton, Sébastien; Calsou, Patrick; Salles, Bernard; Bizard, Anna; Nadal, Marc; Salvati, Erica; Sabatier, Laure; Wu, Yunlin; Biroccio, Annamaria; Londoño-Vallejo, Arturo; Giraud-Panis, Marie Josèphe; Gilson, Eric.

In: Cell, Vol. 142, No. 2, 07.2010, p. 230-242.

Research output: Contribution to journalArticle

Ye, J, Lenain, C, Bauwens, S, Rizzo, A, Saint-Léger, A, Poulet, A, Benarroch, D, Magdinier, F, Morere, J, Amiard, S, Verhoeyen, E, Britton, S, Calsou, P, Salles, B, Bizard, A, Nadal, M, Salvati, E, Sabatier, L, Wu, Y, Biroccio, A, Londoño-Vallejo, A, Giraud-Panis, MJ & Gilson, E 2010, 'TRF2 and Apollo Cooperate with Topoisomerase 2α to Protect Human Telomeres from Replicative Damage', Cell, vol. 142, no. 2, pp. 230-242. https://doi.org/10.1016/j.cell.2010.05.032
Ye J, Lenain C, Bauwens S, Rizzo A, Saint-Léger A, Poulet A et al. TRF2 and Apollo Cooperate with Topoisomerase 2α to Protect Human Telomeres from Replicative Damage. Cell. 2010 Jul;142(2):230-242. https://doi.org/10.1016/j.cell.2010.05.032
Ye, Jing ; Lenain, Christelle ; Bauwens, Serge ; Rizzo, Angela ; Saint-Léger, Adelaïde ; Poulet, Anaïs ; Benarroch, Delphine ; Magdinier, Frédérique ; Morere, Julia ; Amiard, Simon ; Verhoeyen, Els ; Britton, Sébastien ; Calsou, Patrick ; Salles, Bernard ; Bizard, Anna ; Nadal, Marc ; Salvati, Erica ; Sabatier, Laure ; Wu, Yunlin ; Biroccio, Annamaria ; Londoño-Vallejo, Arturo ; Giraud-Panis, Marie Josèphe ; Gilson, Eric. / TRF2 and Apollo Cooperate with Topoisomerase 2α to Protect Human Telomeres from Replicative Damage. In: Cell. 2010 ; Vol. 142, No. 2. pp. 230-242.
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