Abstract
The lack of classical HLA-class I molecules on trophoblast is necessary to prevent allorecognition by maternal CTL, but may induce activation of NK cells. A protective role against NK cells equipped of suitable inhibitory receptors has been proposed for nonclassical HLA-class I molecules including HLA-E and HLA-G. In the present study we show that the NK-mediated killing of two choriocarcinoma cell lines, JAR and JEG3, is induced upon engagement of natural cytotoxicity receptors (NCR) with their specific ligands. In particular, we show that NKp44, a triggering receptor expressed at the NK cell surface only after in vitro culture in the presence of IL-2, plays a central role in triggering NK cytotoxicity against trophoblast cells. Also NKp46 appear to contribute to this function by cooperating with NKp44. On the other hand, other triggering receptors such as NKp30, 2B4, and NKG2D are not involved in killing of choriocarcinoma. Our findings suggest that resistance of trophoblast to NK-mediated cytotoxicity is the result of insufficient activating interactions between the various triggering NK receptors and their target cell ligands. On the other hand, the interaction of nonclassical HLA class I molecules with inhibitory NK receptors appears to play only a marginal role in regulating the susceptibility of choriocarcinoma to NK mediated cytotoxicity.
| Original language | English |
|---|---|
| Pages (from-to) | 1055-1058 |
| Number of pages | 4 |
| Journal | Human Immunology |
| Volume | 61 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - 2000 |
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Keywords
- Natural cytotoxicity
- Triggering receptors
- Trophoblast
ASJC Scopus subject areas
- Immunology
- Immunology and Allergy
Cite this
Triggering receptors involved in natural killer cell-mediated cytotoxicity against choriocarcinoma cell lines. / Sivori, Simona; Parolini, Silvia; Marcenaro, Emanuela; Millo, Romano; Bottino, Cristina; Moretta, Alessandro.
In: Human Immunology, Vol. 61, No. 11, 2000, p. 1055-1058.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Triggering receptors involved in natural killer cell-mediated cytotoxicity against choriocarcinoma cell lines
AU - Sivori, Simona
AU - Parolini, Silvia
AU - Marcenaro, Emanuela
AU - Millo, Romano
AU - Bottino, Cristina
AU - Moretta, Alessandro
PY - 2000
Y1 - 2000
N2 - The lack of classical HLA-class I molecules on trophoblast is necessary to prevent allorecognition by maternal CTL, but may induce activation of NK cells. A protective role against NK cells equipped of suitable inhibitory receptors has been proposed for nonclassical HLA-class I molecules including HLA-E and HLA-G. In the present study we show that the NK-mediated killing of two choriocarcinoma cell lines, JAR and JEG3, is induced upon engagement of natural cytotoxicity receptors (NCR) with their specific ligands. In particular, we show that NKp44, a triggering receptor expressed at the NK cell surface only after in vitro culture in the presence of IL-2, plays a central role in triggering NK cytotoxicity against trophoblast cells. Also NKp46 appear to contribute to this function by cooperating with NKp44. On the other hand, other triggering receptors such as NKp30, 2B4, and NKG2D are not involved in killing of choriocarcinoma. Our findings suggest that resistance of trophoblast to NK-mediated cytotoxicity is the result of insufficient activating interactions between the various triggering NK receptors and their target cell ligands. On the other hand, the interaction of nonclassical HLA class I molecules with inhibitory NK receptors appears to play only a marginal role in regulating the susceptibility of choriocarcinoma to NK mediated cytotoxicity.
AB - The lack of classical HLA-class I molecules on trophoblast is necessary to prevent allorecognition by maternal CTL, but may induce activation of NK cells. A protective role against NK cells equipped of suitable inhibitory receptors has been proposed for nonclassical HLA-class I molecules including HLA-E and HLA-G. In the present study we show that the NK-mediated killing of two choriocarcinoma cell lines, JAR and JEG3, is induced upon engagement of natural cytotoxicity receptors (NCR) with their specific ligands. In particular, we show that NKp44, a triggering receptor expressed at the NK cell surface only after in vitro culture in the presence of IL-2, plays a central role in triggering NK cytotoxicity against trophoblast cells. Also NKp46 appear to contribute to this function by cooperating with NKp44. On the other hand, other triggering receptors such as NKp30, 2B4, and NKG2D are not involved in killing of choriocarcinoma. Our findings suggest that resistance of trophoblast to NK-mediated cytotoxicity is the result of insufficient activating interactions between the various triggering NK receptors and their target cell ligands. On the other hand, the interaction of nonclassical HLA class I molecules with inhibitory NK receptors appears to play only a marginal role in regulating the susceptibility of choriocarcinoma to NK mediated cytotoxicity.
KW - Natural cytotoxicity
KW - Triggering receptors
KW - Trophoblast
UR - http://www.scopus.com/inward/record.url?scp=0034501884&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034501884&partnerID=8YFLogxK
U2 - 10.1016/S0198-8859(00)00201-9
DO - 10.1016/S0198-8859(00)00201-9
M3 - Article
C2 - 11137207
AN - SCOPUS:0034501884
VL - 61
SP - 1055
EP - 1058
JO - Human Immunology
JF - Human Immunology
SN - 0198-8859
IS - 11
ER -