TRIM22 inhibits HIV-1 transcription independently of Its E3 ubiquitin ligase activity, Tat, and NF-κB-responsive long terminal repeat elements

Anna Kajaste-Rudnitski, Sara S. Marelli, Cinzia Pultrone, Thomas Pertel, Pradeep D. Uchil, Nadir Mechti, Walther Mothes, Guido Poli, Jeremy Luban, Elisa Vicenzi

Research output: Contribution to journalArticle

Abstract

Previous studies identified clones of the U937 promonocytic cell line that were either permissive or non-permissive for human immunodeficiency virus type 1 (HIV-1) replication. These clones were investigated further in the search for host restriction factors that could explain their differential capacity to support HIV-1 replication. Among known HIV-1 restriction factors screened, tripartite motif-containing protein 22 (TRIM22) was the only factor constitutively expressed in nonpermissive and absent in permissive U937 cells. Stable TRIM22 knockdown (KD) rescued HIV-1 long-terminal-repeat (LTR)-driven transcription in KD-nonpermis-sive cells to the levels observed in permissive cells. Conversely, transduction-mediated expression of TRIM22 in permissive cells reduced LTR-driven luciferase expression by ~7-fold, supporting a negative role of TRIM22 in HIV-1 transcription. This finding was further confirmed in the human T cell line A3.01 expressing TRIM22. Moreover, overexpression of TRIM22 in 293T cells significantly impaired basal and phorbol myris-tate acetate-ionomycin-induced HIV-1 LTR-driven gene expression, whereas inhibition of tumor necrosis factor alpha-induced viral transcription was a consequence of lower basal expression. In agreement, TRIM22 equally inhibited an LTR construct lacking the tandem NF-κB binding sites. In addition, TRIM22 did not affect Tat-mediated LTR transactivation. Finally, these effects were independent of TRIM22 E3 ubiquitin-ligase activity. In the context of replication-competent virus, significantly higher levels of HIV-1 production were observed in KD-nonpermissive versus control nonpermissive U937 cells after infection. In contrast, lower peak levels of HIV-1 replication characterized U937 and A3.01 cells expressing TRIM22 versus their control transduced counterpart. Thus, nuclear TRIM22 significantly impairs HIV-1 replication, likely by interfering with Tat- and NF-κ B-independent LTR-driven transcription.

Original languageEnglish
Pages (from-to)5183-5196
Number of pages14
JournalJournal of Virology
Volume85
Issue number10
DOIs
Publication statusPublished - May 2011

ASJC Scopus subject areas

  • Immunology
  • Virology

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