TRIM8-driven transcriptomic profile of neural stem cells identified glioma-related nodal genes and pathways

Santina Venuto, Stefano Castellana, Maria Monti, Irene Appolloni, Caterina Fusilli, Carmela Fusco, Piero Pucci, Paolo Malatesta, Tommaso Mazza, Giuseppe Merla, Lucia Micale

Research output: Contribution to journalArticle

Abstract

Background: We recently reported TRIM8, encoding an E3 ubiquitin ligase, as a gene aberrantly expressed in glioblastoma whose expression suppresses cell growth and induces a significant reduction of clonogenic potential in glioblastoma cell lines. Methods: we provided novel insights on TRIM8 functions by profiling the transcriptome of TRIM8-expressing primary mouse embryonal neural stem cells by RNA-sequencing and bioinformatic analysis. Functional analysis including luciferase assay, western blot, PCR arrays, Real time quantitative PCR were performed to validate the transcriptomic data. Results: Our study identified enriched pathways related to the neurotransmission and to the central nervous system (CNS) functions, including axonal guidance, GABA receptor, Ephrin B, synaptic long-term potentiation/depression, and glutamate receptor signalling pathways. Finally, we provided additional evidence about the existence of a functional interactive crosstalk between TRIM8 and STAT3. Conclusions: Our results substantiate the role of TRIM8 in the brain functions through the dysregulation of genes involved in different CNS-related pathways, including JAK-STAT. General significance: This study provides novel insights on the physiological TRIM8 function by profiling for the first time the primary Neural Stem Cell over-expressing TRIM8 by using RNA-Sequencing methodology.

Original languageEnglish
JournalBiochimica et Biophysica Acta - General Subjects
DOIs
Publication statusAccepted/In press - Jan 1 2018

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RNA Sequence Analysis
Neural Stem Cells
Glioblastoma
Stem cells
Glioma
Central Nervous System
Long-Term Synaptic Depression
Genes
Ephrins
GABA-B Receptors
Ubiquitin-Protein Ligases
Long-Term Potentiation
Glutamate Receptors
Neurology
Gene Expression Profiling
Computational Biology
Luciferases
Synaptic Transmission
Real-Time Polymerase Chain Reaction
Western Blotting

Keywords

  • E3 ubiquitin ligase
  • STAT3
  • transcriptome
  • TRIM8

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

Cite this

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title = "TRIM8-driven transcriptomic profile of neural stem cells identified glioma-related nodal genes and pathways",
abstract = "Background: We recently reported TRIM8, encoding an E3 ubiquitin ligase, as a gene aberrantly expressed in glioblastoma whose expression suppresses cell growth and induces a significant reduction of clonogenic potential in glioblastoma cell lines. Methods: we provided novel insights on TRIM8 functions by profiling the transcriptome of TRIM8-expressing primary mouse embryonal neural stem cells by RNA-sequencing and bioinformatic analysis. Functional analysis including luciferase assay, western blot, PCR arrays, Real time quantitative PCR were performed to validate the transcriptomic data. Results: Our study identified enriched pathways related to the neurotransmission and to the central nervous system (CNS) functions, including axonal guidance, GABA receptor, Ephrin B, synaptic long-term potentiation/depression, and glutamate receptor signalling pathways. Finally, we provided additional evidence about the existence of a functional interactive crosstalk between TRIM8 and STAT3. Conclusions: Our results substantiate the role of TRIM8 in the brain functions through the dysregulation of genes involved in different CNS-related pathways, including JAK-STAT. General significance: This study provides novel insights on the physiological TRIM8 function by profiling for the first time the primary Neural Stem Cell over-expressing TRIM8 by using RNA-Sequencing methodology.",
keywords = "E3 ubiquitin ligase, STAT3, transcriptome, TRIM8",
author = "Santina Venuto and Stefano Castellana and Maria Monti and Irene Appolloni and Caterina Fusilli and Carmela Fusco and Piero Pucci and Paolo Malatesta and Tommaso Mazza and Giuseppe Merla and Lucia Micale",
year = "2018",
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doi = "10.1016/j.bbagen.2018.12.001",
language = "English",
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T1 - TRIM8-driven transcriptomic profile of neural stem cells identified glioma-related nodal genes and pathways

AU - Venuto, Santina

AU - Castellana, Stefano

AU - Monti, Maria

AU - Appolloni, Irene

AU - Fusilli, Caterina

AU - Fusco, Carmela

AU - Pucci, Piero

AU - Malatesta, Paolo

AU - Mazza, Tommaso

AU - Merla, Giuseppe

AU - Micale, Lucia

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Background: We recently reported TRIM8, encoding an E3 ubiquitin ligase, as a gene aberrantly expressed in glioblastoma whose expression suppresses cell growth and induces a significant reduction of clonogenic potential in glioblastoma cell lines. Methods: we provided novel insights on TRIM8 functions by profiling the transcriptome of TRIM8-expressing primary mouse embryonal neural stem cells by RNA-sequencing and bioinformatic analysis. Functional analysis including luciferase assay, western blot, PCR arrays, Real time quantitative PCR were performed to validate the transcriptomic data. Results: Our study identified enriched pathways related to the neurotransmission and to the central nervous system (CNS) functions, including axonal guidance, GABA receptor, Ephrin B, synaptic long-term potentiation/depression, and glutamate receptor signalling pathways. Finally, we provided additional evidence about the existence of a functional interactive crosstalk between TRIM8 and STAT3. Conclusions: Our results substantiate the role of TRIM8 in the brain functions through the dysregulation of genes involved in different CNS-related pathways, including JAK-STAT. General significance: This study provides novel insights on the physiological TRIM8 function by profiling for the first time the primary Neural Stem Cell over-expressing TRIM8 by using RNA-Sequencing methodology.

AB - Background: We recently reported TRIM8, encoding an E3 ubiquitin ligase, as a gene aberrantly expressed in glioblastoma whose expression suppresses cell growth and induces a significant reduction of clonogenic potential in glioblastoma cell lines. Methods: we provided novel insights on TRIM8 functions by profiling the transcriptome of TRIM8-expressing primary mouse embryonal neural stem cells by RNA-sequencing and bioinformatic analysis. Functional analysis including luciferase assay, western blot, PCR arrays, Real time quantitative PCR were performed to validate the transcriptomic data. Results: Our study identified enriched pathways related to the neurotransmission and to the central nervous system (CNS) functions, including axonal guidance, GABA receptor, Ephrin B, synaptic long-term potentiation/depression, and glutamate receptor signalling pathways. Finally, we provided additional evidence about the existence of a functional interactive crosstalk between TRIM8 and STAT3. Conclusions: Our results substantiate the role of TRIM8 in the brain functions through the dysregulation of genes involved in different CNS-related pathways, including JAK-STAT. General significance: This study provides novel insights on the physiological TRIM8 function by profiling for the first time the primary Neural Stem Cell over-expressing TRIM8 by using RNA-Sequencing methodology.

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