TY - JOUR
T1 - TRIM8 interacts with KIF11 and KIFC1 and controls bipolar spindle formation and chromosomal stability
AU - Venuto, Santina
AU - Monteonofrio, Laura
AU - Cozzolino, Flora
AU - Monti, Maria
AU - Appolloni, Irene
AU - Mazza, Tommaso
AU - Canetti, Diana
AU - Giambra, Vincenzo
AU - Panelli, Patrizio
AU - Fusco, Carmela
AU - Squeo, Gabriella Maria
AU - Croce, Anna Irma
AU - Pucci, Pietro
AU - Malatesta, Paolo
AU - Soddu, Silvia
AU - Merla, Giuseppe
AU - Micale, Lucia
PY - 2020/3/31
Y1 - 2020/3/31
N2 - The faithful inheritance of chromosomes is essential for the propagation of organisms. In eukaryotes, central to this process is the mitotic spindle. Recently, we have identified TRIM8 as a gene aberrantly expressed in gliomas whose expression reduces the clonogenic potential in the patients' glioma cells. TRIM8 encodes an E3 ubiquitin ligase involved in various pathological processes, including hypertrophy, antiviral defense, encephalopathy, and cancer development. To gain insights into the TRIM8 functions, we characterized the TRIM8 interactome in primary mouse embryonic neural stem cells using proteomics. We found that TRIM8 interacts with KIFC1, and KIF11/Eg5, two master regulators of mitotic spindle assembly and cytoskeleton reorganization. By exploring the TRIM8 role in the mitotic spindle machinery, we showed that TRIM8 localizes at the mitotic spindle during mitosis and plays a role in centrosome separation at the beginning of mitosis with a subsequent delay of the mitotic progression and impact on chromosomal stability.
AB - The faithful inheritance of chromosomes is essential for the propagation of organisms. In eukaryotes, central to this process is the mitotic spindle. Recently, we have identified TRIM8 as a gene aberrantly expressed in gliomas whose expression reduces the clonogenic potential in the patients' glioma cells. TRIM8 encodes an E3 ubiquitin ligase involved in various pathological processes, including hypertrophy, antiviral defense, encephalopathy, and cancer development. To gain insights into the TRIM8 functions, we characterized the TRIM8 interactome in primary mouse embryonic neural stem cells using proteomics. We found that TRIM8 interacts with KIFC1, and KIF11/Eg5, two master regulators of mitotic spindle assembly and cytoskeleton reorganization. By exploring the TRIM8 role in the mitotic spindle machinery, we showed that TRIM8 localizes at the mitotic spindle during mitosis and plays a role in centrosome separation at the beginning of mitosis with a subsequent delay of the mitotic progression and impact on chromosomal stability.
KW - Chromosomal stability
KW - Kinesins
KW - Mitosis
KW - TRIM8
UR - http://www.scopus.com/inward/record.url?scp=85077392222&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85077392222&partnerID=8YFLogxK
U2 - 10.1016/j.canlet.2019.12.042
DO - 10.1016/j.canlet.2019.12.042
M3 - Article
C2 - 31904480
AN - SCOPUS:85077392222
VL - 473
SP - 98
EP - 106
JO - Cancer Letters
JF - Cancer Letters
SN - 0304-3835
ER -