Abstract
High molecular weight hyaluronic acid derivative was prepared in a radioactive form, labelled with tritium. The mucopolysaccharide was oxidized under mild conditions by sodium periodate. After purification the oxidation product was reduced with [3H]NaBH4. The tritium labelled hyaluronic acid derivative was obtained with a high specific radioactivity (0.15 mCi/mg) and was found to have similar physical and chemical characteristics (high molecular weight, infrared spectrum and electrophoretical behaviour) to the parent compound. The radiochemical purity of labelled hyaluronic acid derivative was found to be greater than 95%.
Original language | English |
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Pages (from-to) | 961-969 |
Number of pages | 9 |
Journal | Journal of Labelled Compounds and Radiopharmaceuticals |
Volume | 22 |
Issue number | 9 |
DOIs | |
Publication status | Published - 1985 |
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Radiology Nuclear Medicine and imaging
- Drug Discovery
- Spectroscopy
- Organic Chemistry