Troglitazione affects survival of human osteosarcoma cells

Enrico Lucarelli, Luca Sangiorgi, Veronica Maini, Giovanna Lattanzi, Sandra Marmiroli, Matteo Reggiani, Marina Mordenti, Giuliana Alessandra Gobbi, Francesca Scrimieri, Annarosa Zambon Bertoja, Piero Picci

Research output: Contribution to journalArticlepeer-review

Abstract

Activation of PPARγ, a transcription factor member of the family of peroxisome proliferator-activated receptors, induces apoptosis in several normal and tumor cell lines. In our study, we investigated whether treatment with troglitazone (TRO), a known PPARγ agonist, induced apoptosis in the human osteosarcoma (OS) cell lines G292, MG63, SAOS and U2OS that express PPARγ. In our experiments, TRO never induced apoptosis of OS cells; on the contrary, TRO increased cell number, based on MTT proliferation assay. Remarkably, the TRO-induced cell number increase depended on a decrease of apoptosis that naturally occurred in the culture and was not due to an increased cell proliferation rate. TRO also prevented staurosporin-induced apoptosis. The TRO-mediated survival effect correlated with the activation of Akt, a well-known mediator of survival stimuli. Our work describes a new function for TRO and indicates that the Akt survival pathway may be a mediator of TRO-induced increase of survival.

Original languageEnglish
Pages (from-to)344-351
Number of pages8
JournalInternational Journal of Cancer
Volume98
Issue number3
DOIs
Publication statusPublished - Mar 20 2002

Keywords

  • Apoptosis
  • Nuclear receptors
  • Osteosarcoma
  • Survival

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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