TRPA1 Receptor Induced Relaxation of the Human Urethra Involves TRPV1 and Cannabinoid Receptor Mediated Signals, and Cyclooxygenase Activation

Philipp Weinhold, Christian Gratzke, Tomi Streng, Christian Stief, Karl Erik Andersson, Petter Hedlund

Research output: Contribution to journalArticle

Abstract

Purpose: We studied whether TRPA1 agonists interact with sensory and inflammatory signals to relax human urethral smooth muscle. Materials and Methods: Urethral specimens were obtained perioperatively from 19 patients, and prepared for immunohistochemistry and functional experiments. The effects of allyl isothiocyanate, cinnamaldehyde and NaHS were studied in phenylephrine activated preparations combined with capsaicin, capsazepine, Nω-nitro-L-arginine, indomethacin or CP55940. Results: TRPA1, cannabinoid 1 and cannabinoid 2 immunoreactivity was colocalized in nerve fibers of the human urethra. All TRPA1 agonists produced relaxation of phenylephrine contracted urethral preparations. Capsaicin increased relaxant responses to all TRPA1 agonists. It increased the mean ± SEM -logIC50 of cinnamaldehyde and NaHS from 4.91 ± 0.26 to 5.15 ± 0.22 and 3.27 ± 0.14 to 3.79 ± 0.35, and the -logIC30 of allyl isothiocyanate from 3.11 ± 0.24 to 3.41 ± 0.26 (each p

Original languageEnglish
Pages (from-to)2070-2076
Number of pages7
JournalJournal of Urology
Volume183
Issue number5
DOIs
Publication statusPublished - May 2010

Keywords

  • muscle relaxation
  • TRPA1 protein, human
  • TRPV1 protein, human
  • urethra
  • urothelium

ASJC Scopus subject areas

  • Urology

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