Truffles contain endocannabinoid metabolic enzymes and anandamide

Giovanni Pacioni, Cinzia Rapino, Osvaldo Zarivi, Anastasia Falconi, Marco Leonardi, Natalia Battista, Sabrina Colafarina, Manuel Sergi, Antonella Bonfigli, Michele Miranda, Daniela Barsacchi, Mauro Maccarrone

Research output: Contribution to journalArticle

Abstract

Truffles are the fruiting body of fungi, members of the Ascomycota phylum endowed with major gastronomic and commercial value. The development and maturation of their reproductive structure are dependent on melanin synthesis. Since anandamide, a prominent member of the endocannabinoid system (ECS), is responsible for melanin synthesis in normal human epidermal melanocytes, we thought that ECS might be present also in truffles. Here, we show the expression, at the transcriptional and translational levels, of most ECS components in the black truffle Tuber melanosporum Vittad. at maturation stage VI. Indeed, by means of molecular biology and immunochemical techniques, we found that truffles contain the major metabolic enzymes of the ECS, while they do not express the most relevant endocannabinoid-binding receptors. In addition, we measured anandamide content in truffles, at different maturation stages (from III to VI), through liquid chromatography-mass spectrometric analysis, whereas the other relevant endocannabinoid 2-arachidonoylglycerol was below the detection limit. Overall, our unprecedented results suggest that anandamide and ECS metabolic enzymes have evolved earlier than endocannabinoid-binding receptors, and that anandamide might be an ancient attractant to truffle eaters, that are well-equipped with endocannabinoid-binding receptors.

Original languageEnglish
Pages (from-to)104-110
Number of pages7
JournalPhytochemistry
Volume110
DOIs
Publication statusPublished - 2015

Fingerprint

truffles
Endocannabinoids
Enzymes
enzymes
melanin
receptors
Melanins
Tuber melanosporum
synthesis
melanocytes
attractants
fruiting bodies
Ascomycota
molecular biology
liquid chromatography
anandamide
detection limit
Molecular biology
Melanocytes
Liquid chromatography

Keywords

  • Endocannabinoids
  • Evolution
  • Food intake
  • Maturation
  • Reward
  • Truffle

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry
  • Molecular Biology
  • Horticulture
  • Medicine(all)

Cite this

Pacioni, G., Rapino, C., Zarivi, O., Falconi, A., Leonardi, M., Battista, N., ... Maccarrone, M. (2015). Truffles contain endocannabinoid metabolic enzymes and anandamide. Phytochemistry, 110, 104-110. https://doi.org/10.1016/j.phytochem.2014.11.012

Truffles contain endocannabinoid metabolic enzymes and anandamide. / Pacioni, Giovanni; Rapino, Cinzia; Zarivi, Osvaldo; Falconi, Anastasia; Leonardi, Marco; Battista, Natalia; Colafarina, Sabrina; Sergi, Manuel; Bonfigli, Antonella; Miranda, Michele; Barsacchi, Daniela; Maccarrone, Mauro.

In: Phytochemistry, Vol. 110, 2015, p. 104-110.

Research output: Contribution to journalArticle

Pacioni, G, Rapino, C, Zarivi, O, Falconi, A, Leonardi, M, Battista, N, Colafarina, S, Sergi, M, Bonfigli, A, Miranda, M, Barsacchi, D & Maccarrone, M 2015, 'Truffles contain endocannabinoid metabolic enzymes and anandamide', Phytochemistry, vol. 110, pp. 104-110. https://doi.org/10.1016/j.phytochem.2014.11.012
Pacioni G, Rapino C, Zarivi O, Falconi A, Leonardi M, Battista N et al. Truffles contain endocannabinoid metabolic enzymes and anandamide. Phytochemistry. 2015;110:104-110. https://doi.org/10.1016/j.phytochem.2014.11.012
Pacioni, Giovanni ; Rapino, Cinzia ; Zarivi, Osvaldo ; Falconi, Anastasia ; Leonardi, Marco ; Battista, Natalia ; Colafarina, Sabrina ; Sergi, Manuel ; Bonfigli, Antonella ; Miranda, Michele ; Barsacchi, Daniela ; Maccarrone, Mauro. / Truffles contain endocannabinoid metabolic enzymes and anandamide. In: Phytochemistry. 2015 ; Vol. 110. pp. 104-110.
@article{7232e443c838423896c5155d9ac52a8a,
title = "Truffles contain endocannabinoid metabolic enzymes and anandamide",
abstract = "Truffles are the fruiting body of fungi, members of the Ascomycota phylum endowed with major gastronomic and commercial value. The development and maturation of their reproductive structure are dependent on melanin synthesis. Since anandamide, a prominent member of the endocannabinoid system (ECS), is responsible for melanin synthesis in normal human epidermal melanocytes, we thought that ECS might be present also in truffles. Here, we show the expression, at the transcriptional and translational levels, of most ECS components in the black truffle Tuber melanosporum Vittad. at maturation stage VI. Indeed, by means of molecular biology and immunochemical techniques, we found that truffles contain the major metabolic enzymes of the ECS, while they do not express the most relevant endocannabinoid-binding receptors. In addition, we measured anandamide content in truffles, at different maturation stages (from III to VI), through liquid chromatography-mass spectrometric analysis, whereas the other relevant endocannabinoid 2-arachidonoylglycerol was below the detection limit. Overall, our unprecedented results suggest that anandamide and ECS metabolic enzymes have evolved earlier than endocannabinoid-binding receptors, and that anandamide might be an ancient attractant to truffle eaters, that are well-equipped with endocannabinoid-binding receptors.",
keywords = "Endocannabinoids, Evolution, Food intake, Maturation, Reward, Truffle",
author = "Giovanni Pacioni and Cinzia Rapino and Osvaldo Zarivi and Anastasia Falconi and Marco Leonardi and Natalia Battista and Sabrina Colafarina and Manuel Sergi and Antonella Bonfigli and Michele Miranda and Daniela Barsacchi and Mauro Maccarrone",
year = "2015",
doi = "10.1016/j.phytochem.2014.11.012",
language = "English",
volume = "110",
pages = "104--110",
journal = "Phytochemistry",
issn = "0031-9422",
publisher = "Elsevier Limited",

}

TY - JOUR

T1 - Truffles contain endocannabinoid metabolic enzymes and anandamide

AU - Pacioni, Giovanni

AU - Rapino, Cinzia

AU - Zarivi, Osvaldo

AU - Falconi, Anastasia

AU - Leonardi, Marco

AU - Battista, Natalia

AU - Colafarina, Sabrina

AU - Sergi, Manuel

AU - Bonfigli, Antonella

AU - Miranda, Michele

AU - Barsacchi, Daniela

AU - Maccarrone, Mauro

PY - 2015

Y1 - 2015

N2 - Truffles are the fruiting body of fungi, members of the Ascomycota phylum endowed with major gastronomic and commercial value. The development and maturation of their reproductive structure are dependent on melanin synthesis. Since anandamide, a prominent member of the endocannabinoid system (ECS), is responsible for melanin synthesis in normal human epidermal melanocytes, we thought that ECS might be present also in truffles. Here, we show the expression, at the transcriptional and translational levels, of most ECS components in the black truffle Tuber melanosporum Vittad. at maturation stage VI. Indeed, by means of molecular biology and immunochemical techniques, we found that truffles contain the major metabolic enzymes of the ECS, while they do not express the most relevant endocannabinoid-binding receptors. In addition, we measured anandamide content in truffles, at different maturation stages (from III to VI), through liquid chromatography-mass spectrometric analysis, whereas the other relevant endocannabinoid 2-arachidonoylglycerol was below the detection limit. Overall, our unprecedented results suggest that anandamide and ECS metabolic enzymes have evolved earlier than endocannabinoid-binding receptors, and that anandamide might be an ancient attractant to truffle eaters, that are well-equipped with endocannabinoid-binding receptors.

AB - Truffles are the fruiting body of fungi, members of the Ascomycota phylum endowed with major gastronomic and commercial value. The development and maturation of their reproductive structure are dependent on melanin synthesis. Since anandamide, a prominent member of the endocannabinoid system (ECS), is responsible for melanin synthesis in normal human epidermal melanocytes, we thought that ECS might be present also in truffles. Here, we show the expression, at the transcriptional and translational levels, of most ECS components in the black truffle Tuber melanosporum Vittad. at maturation stage VI. Indeed, by means of molecular biology and immunochemical techniques, we found that truffles contain the major metabolic enzymes of the ECS, while they do not express the most relevant endocannabinoid-binding receptors. In addition, we measured anandamide content in truffles, at different maturation stages (from III to VI), through liquid chromatography-mass spectrometric analysis, whereas the other relevant endocannabinoid 2-arachidonoylglycerol was below the detection limit. Overall, our unprecedented results suggest that anandamide and ECS metabolic enzymes have evolved earlier than endocannabinoid-binding receptors, and that anandamide might be an ancient attractant to truffle eaters, that are well-equipped with endocannabinoid-binding receptors.

KW - Endocannabinoids

KW - Evolution

KW - Food intake

KW - Maturation

KW - Reward

KW - Truffle

UR - http://www.scopus.com/inward/record.url?scp=84921496853&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84921496853&partnerID=8YFLogxK

U2 - 10.1016/j.phytochem.2014.11.012

DO - 10.1016/j.phytochem.2014.11.012

M3 - Article

C2 - 25433633

AN - SCOPUS:84921496853

VL - 110

SP - 104

EP - 110

JO - Phytochemistry

JF - Phytochemistry

SN - 0031-9422

ER -