Trypsin activation of inactive renin in human plasma an assessment of some methodological aspects

A. Rappelli, N. Glorioso, P. Madeddu, P. Dessi-Fulgheri, G. Fois, L. Lenguini, F. Meloni, M. Palermo

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The following methodological aspects of the use of trypsin as activator of inactive renin in human plasma have been studied:a) the effect of SBTI on renin activity and angiotensin;b) the reaction velocity of trypsin on inactive renin;c) the optimum trypsin concentration;d) the ability of human plasma to neutralize exogenous trypsin. Our results show that:1) Some commercially available SBTI may exert an angiotensinase-like effect which can be abolished by PMSF. 2) At 4°C activation of inactive renin reached a maximum within the first two minutes then no further activation could be demonstrated. 3)Trypsin 2 mg/ml yielded more inactive renin than trypsin 1 or 0.5 mg/ml. A higher concentration (3 mg/ml) gave substantially equivalent activation as(with)trypsin 2 mg/ml whereas when using a still higher concentration (4 mg/ml)a degradation of the renin system components could be noted. 4)Endogenous trypsin inhibitors can eventually inactivate exogenous trypsin up to 3 mg/ml. About 20 % of renin is destroyed by trypsin 4 mg/ml within 2 min at 4°C while an additional 40% is lost during the incubation at 37°C if no SBTI is added.

Original languageEnglish
Pages (from-to)299-318
Number of pages20
JournalClinical and Experimental Hypertension
Volume3
Issue number2
DOIs
Publication statusPublished - 1981

Fingerprint

Renin
Trypsin
Trypsin Inhibitors
Angiotensins

ASJC Scopus subject areas

  • Internal Medicine
  • Physiology

Cite this

Trypsin activation of inactive renin in human plasma an assessment of some methodological aspects. / Rappelli, A.; Glorioso, N.; Madeddu, P.; Dessi-Fulgheri, P.; Fois, G.; Lenguini, L.; Meloni, F.; Palermo, M.

In: Clinical and Experimental Hypertension, Vol. 3, No. 2, 1981, p. 299-318.

Research output: Contribution to journalArticle

Rappelli, A, Glorioso, N, Madeddu, P, Dessi-Fulgheri, P, Fois, G, Lenguini, L, Meloni, F & Palermo, M 1981, 'Trypsin activation of inactive renin in human plasma an assessment of some methodological aspects', Clinical and Experimental Hypertension, vol. 3, no. 2, pp. 299-318. https://doi.org/10.3109/10641968109033666
Rappelli, A. ; Glorioso, N. ; Madeddu, P. ; Dessi-Fulgheri, P. ; Fois, G. ; Lenguini, L. ; Meloni, F. ; Palermo, M. / Trypsin activation of inactive renin in human plasma an assessment of some methodological aspects. In: Clinical and Experimental Hypertension. 1981 ; Vol. 3, No. 2. pp. 299-318.
@article{81abc001af00432facdbdfb2aae9ed48,
title = "Trypsin activation of inactive renin in human plasma an assessment of some methodological aspects",
abstract = "The following methodological aspects of the use of trypsin as activator of inactive renin in human plasma have been studied:a) the effect of SBTI on renin activity and angiotensin;b) the reaction velocity of trypsin on inactive renin;c) the optimum trypsin concentration;d) the ability of human plasma to neutralize exogenous trypsin. Our results show that:1) Some commercially available SBTI may exert an angiotensinase-like effect which can be abolished by PMSF. 2) At 4°C activation of inactive renin reached a maximum within the first two minutes then no further activation could be demonstrated. 3)Trypsin 2 mg/ml yielded more inactive renin than trypsin 1 or 0.5 mg/ml. A higher concentration (3 mg/ml) gave substantially equivalent activation as(with)trypsin 2 mg/ml whereas when using a still higher concentration (4 mg/ml)a degradation of the renin system components could be noted. 4)Endogenous trypsin inhibitors can eventually inactivate exogenous trypsin up to 3 mg/ml. About 20 {\%} of renin is destroyed by trypsin 4 mg/ml within 2 min at 4°C while an additional 40{\%} is lost during the incubation at 37°C if no SBTI is added.",
author = "A. Rappelli and N. Glorioso and P. Madeddu and P. Dessi-Fulgheri and G. Fois and L. Lenguini and F. Meloni and M. Palermo",
year = "1981",
doi = "10.3109/10641968109033666",
language = "English",
volume = "3",
pages = "299--318",
journal = "Clinical and Experimental Hypertension",
issn = "1064-1963",
publisher = "Informa Healthcare",
number = "2",

}

TY - JOUR

T1 - Trypsin activation of inactive renin in human plasma an assessment of some methodological aspects

AU - Rappelli, A.

AU - Glorioso, N.

AU - Madeddu, P.

AU - Dessi-Fulgheri, P.

AU - Fois, G.

AU - Lenguini, L.

AU - Meloni, F.

AU - Palermo, M.

PY - 1981

Y1 - 1981

N2 - The following methodological aspects of the use of trypsin as activator of inactive renin in human plasma have been studied:a) the effect of SBTI on renin activity and angiotensin;b) the reaction velocity of trypsin on inactive renin;c) the optimum trypsin concentration;d) the ability of human plasma to neutralize exogenous trypsin. Our results show that:1) Some commercially available SBTI may exert an angiotensinase-like effect which can be abolished by PMSF. 2) At 4°C activation of inactive renin reached a maximum within the first two minutes then no further activation could be demonstrated. 3)Trypsin 2 mg/ml yielded more inactive renin than trypsin 1 or 0.5 mg/ml. A higher concentration (3 mg/ml) gave substantially equivalent activation as(with)trypsin 2 mg/ml whereas when using a still higher concentration (4 mg/ml)a degradation of the renin system components could be noted. 4)Endogenous trypsin inhibitors can eventually inactivate exogenous trypsin up to 3 mg/ml. About 20 % of renin is destroyed by trypsin 4 mg/ml within 2 min at 4°C while an additional 40% is lost during the incubation at 37°C if no SBTI is added.

AB - The following methodological aspects of the use of trypsin as activator of inactive renin in human plasma have been studied:a) the effect of SBTI on renin activity and angiotensin;b) the reaction velocity of trypsin on inactive renin;c) the optimum trypsin concentration;d) the ability of human plasma to neutralize exogenous trypsin. Our results show that:1) Some commercially available SBTI may exert an angiotensinase-like effect which can be abolished by PMSF. 2) At 4°C activation of inactive renin reached a maximum within the first two minutes then no further activation could be demonstrated. 3)Trypsin 2 mg/ml yielded more inactive renin than trypsin 1 or 0.5 mg/ml. A higher concentration (3 mg/ml) gave substantially equivalent activation as(with)trypsin 2 mg/ml whereas when using a still higher concentration (4 mg/ml)a degradation of the renin system components could be noted. 4)Endogenous trypsin inhibitors can eventually inactivate exogenous trypsin up to 3 mg/ml. About 20 % of renin is destroyed by trypsin 4 mg/ml within 2 min at 4°C while an additional 40% is lost during the incubation at 37°C if no SBTI is added.

UR - http://www.scopus.com/inward/record.url?scp=0019457195&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019457195&partnerID=8YFLogxK

U2 - 10.3109/10641968109033666

DO - 10.3109/10641968109033666

M3 - Article

C2 - 7011711

AN - SCOPUS:0019457195

VL - 3

SP - 299

EP - 318

JO - Clinical and Experimental Hypertension

JF - Clinical and Experimental Hypertension

SN - 1064-1963

IS - 2

ER -