Abstract
The following methodological aspects of the use of trypsin as activator of inactive renin in human plasma have been studied:a) the effect of SBTI on renin activity and angiotensin;b) the reaction velocity of trypsin on inactive renin;c) the optimum trypsin concentration;d) the ability of human plasma to neutralize exogenous trypsin. Our results show that:1) Some commercially available SBTI may exert an angiotensinase-like effect which can be abolished by PMSF. 2) At 4°C activation of inactive renin reached a maximum within the first two minutes then no further activation could be demonstrated. 3)Trypsin 2 mg/ml yielded more inactive renin than trypsin 1 or 0.5 mg/ml. A higher concentration (3 mg/ml) gave substantially equivalent activation as(with)trypsin 2 mg/ml whereas when using a still higher concentration (4 mg/ml)a degradation of the renin system components could be noted. 4)Endogenous trypsin inhibitors can eventually inactivate exogenous trypsin up to 3 mg/ml. About 20 % of renin is destroyed by trypsin 4 mg/ml within 2 min at 4°C while an additional 40% is lost during the incubation at 37°C if no SBTI is added.
| Original language | English |
|---|---|
| Pages (from-to) | 299-318 |
| Number of pages | 20 |
| Journal | Clinical and Experimental Hypertension |
| Volume | 3 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1981 |
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ASJC Scopus subject areas
- Internal Medicine
- Physiology
Cite this
Trypsin activation of inactive renin in human plasma an assessment of some methodological aspects. / Rappelli, A.; Glorioso, N.; Madeddu, P.; Dessi-Fulgheri, P.; Fois, G.; Lenguini, L.; Meloni, F.; Palermo, M.
In: Clinical and Experimental Hypertension, Vol. 3, No. 2, 1981, p. 299-318.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Trypsin activation of inactive renin in human plasma an assessment of some methodological aspects
AU - Rappelli, A.
AU - Glorioso, N.
AU - Madeddu, P.
AU - Dessi-Fulgheri, P.
AU - Fois, G.
AU - Lenguini, L.
AU - Meloni, F.
AU - Palermo, M.
PY - 1981
Y1 - 1981
N2 - The following methodological aspects of the use of trypsin as activator of inactive renin in human plasma have been studied:a) the effect of SBTI on renin activity and angiotensin;b) the reaction velocity of trypsin on inactive renin;c) the optimum trypsin concentration;d) the ability of human plasma to neutralize exogenous trypsin. Our results show that:1) Some commercially available SBTI may exert an angiotensinase-like effect which can be abolished by PMSF. 2) At 4°C activation of inactive renin reached a maximum within the first two minutes then no further activation could be demonstrated. 3)Trypsin 2 mg/ml yielded more inactive renin than trypsin 1 or 0.5 mg/ml. A higher concentration (3 mg/ml) gave substantially equivalent activation as(with)trypsin 2 mg/ml whereas when using a still higher concentration (4 mg/ml)a degradation of the renin system components could be noted. 4)Endogenous trypsin inhibitors can eventually inactivate exogenous trypsin up to 3 mg/ml. About 20 % of renin is destroyed by trypsin 4 mg/ml within 2 min at 4°C while an additional 40% is lost during the incubation at 37°C if no SBTI is added.
AB - The following methodological aspects of the use of trypsin as activator of inactive renin in human plasma have been studied:a) the effect of SBTI on renin activity and angiotensin;b) the reaction velocity of trypsin on inactive renin;c) the optimum trypsin concentration;d) the ability of human plasma to neutralize exogenous trypsin. Our results show that:1) Some commercially available SBTI may exert an angiotensinase-like effect which can be abolished by PMSF. 2) At 4°C activation of inactive renin reached a maximum within the first two minutes then no further activation could be demonstrated. 3)Trypsin 2 mg/ml yielded more inactive renin than trypsin 1 or 0.5 mg/ml. A higher concentration (3 mg/ml) gave substantially equivalent activation as(with)trypsin 2 mg/ml whereas when using a still higher concentration (4 mg/ml)a degradation of the renin system components could be noted. 4)Endogenous trypsin inhibitors can eventually inactivate exogenous trypsin up to 3 mg/ml. About 20 % of renin is destroyed by trypsin 4 mg/ml within 2 min at 4°C while an additional 40% is lost during the incubation at 37°C if no SBTI is added.
UR - http://www.scopus.com/inward/record.url?scp=0019457195&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0019457195&partnerID=8YFLogxK
U2 - 10.3109/10641968109033666
DO - 10.3109/10641968109033666
M3 - Article
C2 - 7011711
AN - SCOPUS:0019457195
VL - 3
SP - 299
EP - 318
JO - Clinical and Experimental Hypertension
JF - Clinical and Experimental Hypertension
SN - 1064-1963
IS - 2
ER -