Tryptase and chymase are angiogenic in vivo in the chorioallantoic membrane assay

Domenico Ribatti, Girolamo Ranieri, Beatrice Nico, Vincenzo Benagiano, Enrico Crivellato

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Human mast cells (MCs) are divided in two types depending on the expression of tryptase and chymase in their granules. Literature data indicate that both tryptase and chymase are angiogenic, but there is currently no evidence of their direct angiogenic activity in vivo. In this study, we have investigated the capacity of tryptase and chymase to promote vasoproliferation in chick embryo chorioallantoic membrane (CAM), a well established in vivo assay to study angiogenesis and anti-angiogenesis. The results showed that both tryptase and chymase stimulate angiogenesis and that the response is similar to that obtained with vascular endothelial growth factor (VEGF), a well-known angiogenic cytokine, and confirm the angiogenic activity of these two proteases stored in MC granules.

Original languageEnglish
Pages (from-to)99-102
Number of pages4
JournalInternational Journal of Developmental Biology
Issue number1
Publication statusPublished - 2011


  • Angiogenesis
  • Chorioallantoic membrane
  • Chymase
  • Mast cells
  • Tryptase

ASJC Scopus subject areas

  • Developmental Biology
  • Embryology


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