TSH/cAMP up-regulate sarco/endoplasmic reticulum Ca2+-ATPases expression and activity in PC Cl3 thyroid cells

Luca Ulianich, Agnese Secondo, Stefania De Micheli, Sonia Treglia, Francesco Pacifico, Domenico Liguoro, Fortunato Moscato, Santo Marsigliante, Lucio Annunziato, Silvestro Formisano, Eduardo Consiglio, Bruno Di Jeso

Research output: Contribution to journalArticle

Abstract

Objective: We recently reported that the sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) 2b is the SERCA form preferentially expressed in rat thyroid. Moreover, SERCA2b expression dramatically decreases in virally transformed, highly tumorigenic, PC Cl3 thyroid cells. These results suggest that, in the thyroid, SERCA2b, in addition to its housekeeping role, is linked to differentiation and is a regulated gene. We therefore sought to study the effect of TSH, the main regulator of thyroid function, on SERCA2b expression and activity. Methods: PC Cl3 cells were hormone starved in low-serum medium and stimulated for long (48 h) or short (1, 2 and 4 h) times. SERCA2b expression and activity were evaluated by Northern and Western blots, Ca2+-ATPase activity and Ca2+ store content. Results: In PC Cl3 cells, SERCA2b mRNA and protein were induced twofold by a 48-h long treatment with TSH. Long-term elevation (48 h) of intracellular cAMP levels, by forskolin or 8-Br-cAMP, had similar effects on SERCA2b mRNA and protein. We also measured Ca2+-ATPase activity and Ca2+ store content. Both long (48 h) and short (0.5-1 h) treatments with TSH, forskolin or 8-Br-cAMP induced a marked increase of SERCA2b activity. This effect was completely abolished by H89, a specific inhibitor of cAMP-dependent protein kinase A (PKA). TSH and 8-Br-cAMP increased Ca2+ store content after both long (48 h) and short (1-2 h) treatments. Conclusions: These data suggested that TSH/cAMP acts as an important regulator of both SERCA2b expression and activity in the thyroid system, through PKA activation.

Original languageEnglish
Pages (from-to)851-861
Number of pages11
JournalEuropean Journal of Endocrinology
Volume150
Issue number6
DOIs
Publication statusPublished - Jun 2004

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Sarcoplasmic Reticulum Calcium-Transporting ATPases
Thyroid Gland
Up-Regulation
Cyclic AMP-Dependent Protein Kinases
Calcium-Transporting ATPases
Colforsin
Housekeeping
Messenger RNA
Serum-Free Culture Media
Northern Blotting
Proteins
Western Blotting
Hormones
Genes

ASJC Scopus subject areas

  • Endocrinology

Cite this

Ulianich, L., Secondo, A., De Micheli, S., Treglia, S., Pacifico, F., Liguoro, D., ... Di Jeso, B. (2004). TSH/cAMP up-regulate sarco/endoplasmic reticulum Ca2+-ATPases expression and activity in PC Cl3 thyroid cells. European Journal of Endocrinology, 150(6), 851-861. https://doi.org/10.1530/eje.0.1500851

TSH/cAMP up-regulate sarco/endoplasmic reticulum Ca2+-ATPases expression and activity in PC Cl3 thyroid cells. / Ulianich, Luca; Secondo, Agnese; De Micheli, Stefania; Treglia, Sonia; Pacifico, Francesco; Liguoro, Domenico; Moscato, Fortunato; Marsigliante, Santo; Annunziato, Lucio; Formisano, Silvestro; Consiglio, Eduardo; Di Jeso, Bruno.

In: European Journal of Endocrinology, Vol. 150, No. 6, 06.2004, p. 851-861.

Research output: Contribution to journalArticle

Ulianich, L, Secondo, A, De Micheli, S, Treglia, S, Pacifico, F, Liguoro, D, Moscato, F, Marsigliante, S, Annunziato, L, Formisano, S, Consiglio, E & Di Jeso, B 2004, 'TSH/cAMP up-regulate sarco/endoplasmic reticulum Ca2+-ATPases expression and activity in PC Cl3 thyroid cells', European Journal of Endocrinology, vol. 150, no. 6, pp. 851-861. https://doi.org/10.1530/eje.0.1500851
Ulianich, Luca ; Secondo, Agnese ; De Micheli, Stefania ; Treglia, Sonia ; Pacifico, Francesco ; Liguoro, Domenico ; Moscato, Fortunato ; Marsigliante, Santo ; Annunziato, Lucio ; Formisano, Silvestro ; Consiglio, Eduardo ; Di Jeso, Bruno. / TSH/cAMP up-regulate sarco/endoplasmic reticulum Ca2+-ATPases expression and activity in PC Cl3 thyroid cells. In: European Journal of Endocrinology. 2004 ; Vol. 150, No. 6. pp. 851-861.
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T1 - TSH/cAMP up-regulate sarco/endoplasmic reticulum Ca2+-ATPases expression and activity in PC Cl3 thyroid cells

AU - Ulianich, Luca

AU - Secondo, Agnese

AU - De Micheli, Stefania

AU - Treglia, Sonia

AU - Pacifico, Francesco

AU - Liguoro, Domenico

AU - Moscato, Fortunato

AU - Marsigliante, Santo

AU - Annunziato, Lucio

AU - Formisano, Silvestro

AU - Consiglio, Eduardo

AU - Di Jeso, Bruno

PY - 2004/6

Y1 - 2004/6

N2 - Objective: We recently reported that the sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) 2b is the SERCA form preferentially expressed in rat thyroid. Moreover, SERCA2b expression dramatically decreases in virally transformed, highly tumorigenic, PC Cl3 thyroid cells. These results suggest that, in the thyroid, SERCA2b, in addition to its housekeeping role, is linked to differentiation and is a regulated gene. We therefore sought to study the effect of TSH, the main regulator of thyroid function, on SERCA2b expression and activity. Methods: PC Cl3 cells were hormone starved in low-serum medium and stimulated for long (48 h) or short (1, 2 and 4 h) times. SERCA2b expression and activity were evaluated by Northern and Western blots, Ca2+-ATPase activity and Ca2+ store content. Results: In PC Cl3 cells, SERCA2b mRNA and protein were induced twofold by a 48-h long treatment with TSH. Long-term elevation (48 h) of intracellular cAMP levels, by forskolin or 8-Br-cAMP, had similar effects on SERCA2b mRNA and protein. We also measured Ca2+-ATPase activity and Ca2+ store content. Both long (48 h) and short (0.5-1 h) treatments with TSH, forskolin or 8-Br-cAMP induced a marked increase of SERCA2b activity. This effect was completely abolished by H89, a specific inhibitor of cAMP-dependent protein kinase A (PKA). TSH and 8-Br-cAMP increased Ca2+ store content after both long (48 h) and short (1-2 h) treatments. Conclusions: These data suggested that TSH/cAMP acts as an important regulator of both SERCA2b expression and activity in the thyroid system, through PKA activation.

AB - Objective: We recently reported that the sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) 2b is the SERCA form preferentially expressed in rat thyroid. Moreover, SERCA2b expression dramatically decreases in virally transformed, highly tumorigenic, PC Cl3 thyroid cells. These results suggest that, in the thyroid, SERCA2b, in addition to its housekeeping role, is linked to differentiation and is a regulated gene. We therefore sought to study the effect of TSH, the main regulator of thyroid function, on SERCA2b expression and activity. Methods: PC Cl3 cells were hormone starved in low-serum medium and stimulated for long (48 h) or short (1, 2 and 4 h) times. SERCA2b expression and activity were evaluated by Northern and Western blots, Ca2+-ATPase activity and Ca2+ store content. Results: In PC Cl3 cells, SERCA2b mRNA and protein were induced twofold by a 48-h long treatment with TSH. Long-term elevation (48 h) of intracellular cAMP levels, by forskolin or 8-Br-cAMP, had similar effects on SERCA2b mRNA and protein. We also measured Ca2+-ATPase activity and Ca2+ store content. Both long (48 h) and short (0.5-1 h) treatments with TSH, forskolin or 8-Br-cAMP induced a marked increase of SERCA2b activity. This effect was completely abolished by H89, a specific inhibitor of cAMP-dependent protein kinase A (PKA). TSH and 8-Br-cAMP increased Ca2+ store content after both long (48 h) and short (1-2 h) treatments. Conclusions: These data suggested that TSH/cAMP acts as an important regulator of both SERCA2b expression and activity in the thyroid system, through PKA activation.

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