TSPAN5 Enriched Microdomains Provide a Platform for Dendritic Spine Maturation through Neuroligin-1 Clustering

Edoardo Moretto, Anna Longatti, Luca Murru, Ingrid Chamma, Alessandro Sessa, Jonathan Zapata, Eric Hosy, Matthieu Sainlos, Julien Saint-Pol, Eric Rubinstein, Daniel Choquet, Vania Broccoli, Giampietro Schiavo, Olivier Thoumine, Maria Passafaro

Research output: Contribution to journalArticle

Abstract

Tetraspanins are a class of evolutionarily conserved transmembrane proteins with 33 members identified in mammals that have the ability to organize specific membrane domains, named tetraspanin-enriched microdomains (TEMs). Despite the relative abundance of different tetraspanins in the CNS, few studies have explored their role at synapses. Here, we investigate the function of TSPAN5, a member of the tetraspanin superfamily for which mRNA transcripts are found at high levels in the mouse brain. We demonstrate that TSPAN5 is localized in dendritic spines of pyramidal excitatory neurons and that TSPAN5 knockdown induces a dramatic decrease in spine number because of defects in the spine maturation process. Moreover, we show that TSPAN5 interacts with the postsynaptic adhesion molecule neuroligin-1, promoting its correct surface clustering. We propose that membrane compartmentalization by tetraspanins represents an additional mechanism for regulating excitatory synapses.

Original languageEnglish
Pages (from-to)1130-1146.e8
JournalCell Reports
Volume29
Issue number5
DOIs
Publication statusPublished - Oct 29 2019

Keywords

  • clustering
  • dendritic spines
  • neuroligin-1
  • synapses
  • TEMs
  • tetraspanin
  • TSPAN5

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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  • Cite this

    Moretto, E., Longatti, A., Murru, L., Chamma, I., Sessa, A., Zapata, J., Hosy, E., Sainlos, M., Saint-Pol, J., Rubinstein, E., Choquet, D., Broccoli, V., Schiavo, G., Thoumine, O., & Passafaro, M. (2019). TSPAN5 Enriched Microdomains Provide a Platform for Dendritic Spine Maturation through Neuroligin-1 Clustering. Cell Reports, 29(5), 1130-1146.e8. https://doi.org/10.1016/j.celrep.2019.09.051