TSPO interacts with VDAC1 and triggers a ROS-mediated inhibition of mitochondrial quality control

Jemma Gatliff, Daniel East, James Crosby, Rosella Abeti, Robert Harvey, William Craigen, Peter Parker, Michelangelo Campanella

Research output: Contribution to journalArticlepeer-review

Abstract

The 18-kDa TSPO (translocator protein) localizes on the outer mitochondrial membrane (OMM) and participates in cholesterol transport. Here, we report that TSPO inhibits mitochondrial autophagy downstream of the PINK1-PARK2 pathway, preventing essential ubiquitination of proteins. TSPO abolishes mitochondrial relocation of SQSTM1/p62 (sequestosome 1), and consequently that of the autophagic marker LC3 (microtubule-associated protein 1 light chain 3), thus leading to an accumulation of dysfunctional mitochondria, altering the appearance of the network. Independent of cholesterol regulation, the modulation of mitophagy by TSPO is instead dependent on VDAC1 (voltagedependent anion channel 1), to which TSPO binds, reducing mitochondrial coupling and promoting an overproduction of reactive oxygen species (ROS) that counteracts PARK2-mediated ubiquitination of proteins. These data identify TSPO as a novel element in the regulation of mitochondrial quality control by autophagy, and demonstrate the importance for cell homeostasis of its expression ratio with VDAC1.

Original languageEnglish
Pages (from-to)2279-2296
Number of pages18
JournalAutophagy
Volume10
Issue number12
DOIs
Publication statusPublished - Dec 1 2014

Keywords

  • Mitochondria
  • Mitophagy
  • PARK2
  • ROS
  • TSPO
  • Ubiquitin

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

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