TTF-1, cytokeratin 7, 34βE12, and CD56/NCAM immunostaining in the subclassification of large cell carcinomas of the lung

Giulio Rossi, Alessandro Marchioni, Marina Milani, Rosa Scotti, Moira Foroni, AnnaMaria Cesinaro, Lucia Longo, Mario Migaldi, Alberto Cavazza

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

We selected a 4-stain immunopanel including thyroid transcription factor (TTF)-1, cytokeratin (CK) 7, 34βE12, and CD56/neural cell adhesion molecule (NCAM) to subclassify a series of 45 pulmonary large cell carcinomas (LCCs) on bronchial biopsy. All cases consisted of a large tumor cell proliferation with abundant cytoplasm, vesicular nuclei, and prominent nucleoli. Immunohistochemically, 27 tumors (60%) were subclassified as adenocarcinoma (TTF-1+/CK7+, 24; CK7+ only, 3), 10 (22%) as squamous cell carcinoma (34βE12+ only), and 4 (9%) as LCC with neuroendocrine differentiation (CD56+, variably stained with TTF-1 and CK7, 34βE12-). In 4 cases, the tumors coexpressed CK7 and 34βE12 (3 cases) or were completely unstained (1 case). Surgically resected tumors matched exactly with the corresponding original biopsy specimens in 21 of 23 cases; consistent CD56 expression was a reliable marker in confirming a diagnosis of large cell neuroendocrine carcinoma even on biopsy. Our results suggest that the proposed 4-stain set of commercially available markers might help subclassify LCC even in small biopsy material, validating expression-profiling studies aimed at lung cancer classification and permitting more consistent patient enrollment for trials with targeted treatments.

Original languageEnglish
Pages (from-to)884-893
Number of pages10
JournalAmerican Journal of Clinical Pathology
Volume122
Issue number6
DOIs
Publication statusPublished - Dec 2004

Fingerprint

Keratin-7
Large Cell Carcinoma
Neural Cell Adhesion Molecules
Biopsy
Lung
Neoplasms
Coloring Agents
Neuroendocrine Carcinoma
Squamous Cell Carcinoma
Lung Neoplasms
Cytoplasm
Adenocarcinoma
Cell Proliferation
thyroid nuclear factor 1

Keywords

  • CD56
  • Classification
  • Cytokeratins
  • Gene expression
  • Immunohistochemistry
  • Large cell carcinoma
  • Lung
  • Thyroid transcription factor-1
  • TTF-1

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

TTF-1, cytokeratin 7, 34βE12, and CD56/NCAM immunostaining in the subclassification of large cell carcinomas of the lung. / Rossi, Giulio; Marchioni, Alessandro; Milani, Marina; Scotti, Rosa; Foroni, Moira; Cesinaro, AnnaMaria; Longo, Lucia; Migaldi, Mario; Cavazza, Alberto.

In: American Journal of Clinical Pathology, Vol. 122, No. 6, 12.2004, p. 884-893.

Research output: Contribution to journalArticle

Rossi, Giulio ; Marchioni, Alessandro ; Milani, Marina ; Scotti, Rosa ; Foroni, Moira ; Cesinaro, AnnaMaria ; Longo, Lucia ; Migaldi, Mario ; Cavazza, Alberto. / TTF-1, cytokeratin 7, 34βE12, and CD56/NCAM immunostaining in the subclassification of large cell carcinomas of the lung. In: American Journal of Clinical Pathology. 2004 ; Vol. 122, No. 6. pp. 884-893.
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AU - Rossi, Giulio

AU - Marchioni, Alessandro

AU - Milani, Marina

AU - Scotti, Rosa

AU - Foroni, Moira

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AU - Longo, Lucia

AU - Migaldi, Mario

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AB - We selected a 4-stain immunopanel including thyroid transcription factor (TTF)-1, cytokeratin (CK) 7, 34βE12, and CD56/neural cell adhesion molecule (NCAM) to subclassify a series of 45 pulmonary large cell carcinomas (LCCs) on bronchial biopsy. All cases consisted of a large tumor cell proliferation with abundant cytoplasm, vesicular nuclei, and prominent nucleoli. Immunohistochemically, 27 tumors (60%) were subclassified as adenocarcinoma (TTF-1+/CK7+, 24; CK7+ only, 3), 10 (22%) as squamous cell carcinoma (34βE12+ only), and 4 (9%) as LCC with neuroendocrine differentiation (CD56+, variably stained with TTF-1 and CK7, 34βE12-). In 4 cases, the tumors coexpressed CK7 and 34βE12 (3 cases) or were completely unstained (1 case). Surgically resected tumors matched exactly with the corresponding original biopsy specimens in 21 of 23 cases; consistent CD56 expression was a reliable marker in confirming a diagnosis of large cell neuroendocrine carcinoma even on biopsy. Our results suggest that the proposed 4-stain set of commercially available markers might help subclassify LCC even in small biopsy material, validating expression-profiling studies aimed at lung cancer classification and permitting more consistent patient enrollment for trials with targeted treatments.

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