Tumor Angiogenesis: Methods to analyze tumor vasculature and vessel normalization in mouse models of cancer

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Angiogenesis, the formation of neo-vessels, is one of the most important hallmarks of cancer. Tumor vasculature presents structural abnormalities such as dilatation of vessel diameter and hyper-branched and twisted pattern. A promising strategy in anticancer therapy to overcome the resistance to certain antiangiogenic therapies is the tumor blood vessel normalization, which restores the physiological perfusion and oxygenation of tumor vasculature. Many studies showed how vessel normalization is able to counteract metastasis formation and to favor chemotherapeutic drugs delivery to tumors. Herein we describe different techniques and protocols that allow the study, in vivo the main features of tumor vessel normalization such as the reduction of vessel branching and diameter, the increase of pericyte coverage, the reduction of vessel permeability, and the improvement of vessel perfusion and drug delivery.

Original languageEnglish
Title of host publicationMouse Models of Cancer: Methods and Protocols
PublisherSpringer New York
Pages349-365
Number of pages17
ISBN (Print)9781493922970, 9781493922963
DOIs
Publication statusPublished - Jan 30 2015

Fingerprint

angiogenesis
animal models
neoplasms
Neoplasms
Perfusion
Vascular Tissue Neoplasms
methodology
Pericytes
drugs
therapeutics
Pharmaceutical Preparations
Dilatation
in vivo studies
Permeability
blood vessels
metastasis
branching
permeability
Neoplasm Metastasis
Therapeutics

Keywords

  • Blood vessel normalization
  • Cancer
  • Confocal microscopy
  • Immunofl uorescence
  • Immunohistochemistry
  • Tumor angiogenesis

ASJC Scopus subject areas

  • Medicine(all)
  • Agricultural and Biological Sciences(all)

Cite this

Tumor Angiogenesis : Methods to analyze tumor vasculature and vessel normalization in mouse models of cancer. / Maione, Federica; Giraudo, Enrico.

Mouse Models of Cancer: Methods and Protocols. Springer New York, 2015. p. 349-365.

Research output: Chapter in Book/Report/Conference proceedingChapter

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