To see whether the two methylcholanthrene-induced BALB/c sarcomas C-1 and ST2 expressed H-2 or non-H-2-like alien antigens, either BALB/c or F1 hybrids between BALB/c and other proper strains were immunized by skin grafts followed by a booster of lymphoid cells and challenged with a lethal dose of either sarcoma cells. It was found that all H-2(k) but not H-2(b) tissues elicited a protective immunity to the challenge of C-1; immunization with H-2 different congenic tissues (BALB.K) showed the involvement of genes of the major histocompatibility complex and further experiments allowed the identification of K(k), D(k), and possible I(k) regions as including genes coding for the alien antigens of sarcoma C-1. Immunization of BALB/c hosts or hybrids with tissues differing only for non-H-2 gene products led to the conclusion that ST2 sarcoma expressed minor histocompatibility antigens of c3Hf, A, DBA/2, and B10 backgrounds, whereas C-1 displayed antigens cross-reacting with A but not with C3Hf or B10 tissues. By immunizing BALB/c mice with tissues of a series of B10 or A congenic and recombinant mice, we concluded that the immunogenicity of either B10 or A minor histocompatibility antigens in eliciting an anti-ST2 protective effect was controlled by genes of the D(d) region, which appeared to function as a restriction factor.
|Number of pages||7|
|Journal||Journal of Immunology|
|Publication status||Published - 1980|
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