Tumor-induced expansion of regulatory T cells by conversion of CD4+CD25- lymphocytes is thymus and proliferation independent

Barbara Valzasina, Silvia Piconese, Cristiana Guiducci, Mario P. Colombo

Research output: Contribution to journalArticle

194 Citations (Scopus)

Abstract

The CD25- and CD25+ CD4 T-lymphocyte compartments are tightly regulated. We show here that tumors break such balance, increasing the number of CD4+CD25+ T cells in draining lymph node and spleen but not contralateral node of tumor-bearing mice. Tumor injection in thymectomized and CD25-depleted mice shows that CD4+CD25+ T-cell expansion occurs even in the absence of the thymus and independently from proliferation of preexisting CD25+ T cells. These newly generated cells are bona fide regulatory T cells (T reg) in terms of Foxp3 expression and suppression of CD3-stimulated or allogeneic effector cell proliferation. Transfer of congenic Thy1.1 CD4+CD25- T cells, from mice treated or not with vinblastine, into tumor-bearing or tumor-free mice and analysis of recovered donor lymphocytes indicate that conversion is the main mechanism for acquiring the expression of CD25 and Foxp3 through a process that does not require proliferation. Although conversion of CD4+CD25- T cells for generation of T regs has been described as a natural process that maintains peripheral T-reg population, this process is used by the tumor for immune escape. The prompt recovery of T regs from monoclonal antibody-mediated CD25 depletion in tumor-bearing mice suggests attempts able to inactivate rather than deplete them when treating existing tumors.

Original languageEnglish
Pages (from-to)4488-4495
Number of pages8
JournalCancer Research
Volume66
Issue number8
DOIs
Publication statusPublished - Apr 15 2006

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Regulatory T-Lymphocytes
Thymus Gland
Lymphocytes
T-Lymphocytes
Neoplasms
Tumor Escape
Vinblastine
Spleen
Lymph Nodes
Monoclonal Antibodies
Cell Proliferation
Injections
Population

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Tumor-induced expansion of regulatory T cells by conversion of CD4+CD25- lymphocytes is thymus and proliferation independent. / Valzasina, Barbara; Piconese, Silvia; Guiducci, Cristiana; Colombo, Mario P.

In: Cancer Research, Vol. 66, No. 8, 15.04.2006, p. 4488-4495.

Research output: Contribution to journalArticle

Valzasina, Barbara ; Piconese, Silvia ; Guiducci, Cristiana ; Colombo, Mario P. / Tumor-induced expansion of regulatory T cells by conversion of CD4+CD25- lymphocytes is thymus and proliferation independent. In: Cancer Research. 2006 ; Vol. 66, No. 8. pp. 4488-4495.
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