The authors report their data regarding their experience of in vitro culture and expansion of Tumor Infiltrating Lymphocytes (TIL) deriving from surgically removed melanoma metastases. Twenty out of 32 in vitro cultures showed good expansion levels and the description of their biological data phenotype, number of days in culture, expansion index, cytotoxic activity, are in keeping with data reported in the literature. Thirteen patients received their TIL together with IL-2 in continuous infusion on adoptive immunotherapy regimens. Three patients with advanced disease rapidly progressed. Only one out of 10 disease-free patients progressed in the first 4 months after TIL reinfusion. Nine patients are still disease-free after a median follow-up of 6+ months (range 1 ± 27+). A greater number of patients and a longer follow-up are necessary in order to verify the possible efficacy of adoptive immunotherapy and adjuvant treatment after metastasectomy in melanoma. However, TIL culture experience could be used as a tool for the application of new therapeutic technologies such as gene-transfection.
|Translated title of the contribution||Tumor Infiltrating Lymphocytes (TIL) culture and expansion from melanoma. Adoptive immunotherapy trial with IL-2 in 13 patients. Preliminary data|
|Number of pages||6|
|Journal||Giornale Italiano di Dermatologia e Venereologia|
|Publication status||Published - 1997|
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