Tumor markers in squamous cell carcinoma of esophagus

Immunometric assay in cytosol and membrane fraction

M. Gion, C. Tremolada, R. Mione, R. Dittadi, D. P. Palma, C. Castoro, A. Ruol, A. Nosadini, A. Peracchia, G. Bruscagnin

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Carcinoembryonic antigen (CEA), tissue polypeptide antigen (TPA), ferritin, and the monoclonal antibody-detected tumor-associated antigens CA19.9 and CA50 were measured by radioimmunoassay in tissue fractions of carcinoma and normal esophageal mucosa from 59 patients with untreated primary squamous cell carcinoma of the esophagus. Tumor markers were measured in cytosol (118 samples) and in a membrane-enriched fraction (32 samples). CEA, TPA and ferritin were detected in almost all the cytosol samples evaluated, CA19.9 and CA50 in 66% and 50% of cases respectively. Ferritin was significantly higher in carcinoma than in normal mucosa. The cytosol concentrations of CEA, TPA, CA19.9 and CA50 were not significantly different in carcinoma and normal tissue. Concentrations of CEA, CA19.9 and CA50 in the membrane fraction tended to be higher in normal tissue than in carcinoma whereas the cytosol-to-membrane ratio was significantly higher in carcinoma. For CEA, CA19.9 and CA50, the phenotypic pattern of the malignant transformation seems to involve a different intracellular distribution rather than a quantitative change. No correlations were found between tissue and serum concentrations of the tumor markers, the former being related to the phenotypic characteristics of the tumor, the latter to the tumor burden.

Original languageEnglish
Pages (from-to)7-13
Number of pages7
JournalInternational Journal of Biological Markers
Volume5
Issue number1
Publication statusPublished - 1990

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Carcinoembryonic Antigen
Tumor Biomarkers
Cytosol
Esophagus
Tissue Polypeptide Antigen
Squamous Cell Carcinoma
Assays
Membranes
Carcinoma
Ferritins
Tissue
Tumors
Neoplasm Antigens
Tumor Burden
Radioimmunoassay
Mucous Membrane
Monoclonal Antibodies
Epithelial Cells
Antigens
Serum

ASJC Scopus subject areas

  • Immunology
  • Biochemistry

Cite this

Tumor markers in squamous cell carcinoma of esophagus : Immunometric assay in cytosol and membrane fraction. / Gion, M.; Tremolada, C.; Mione, R.; Dittadi, R.; Palma, D. P.; Castoro, C.; Ruol, A.; Nosadini, A.; Peracchia, A.; Bruscagnin, G.

In: International Journal of Biological Markers, Vol. 5, No. 1, 1990, p. 7-13.

Research output: Contribution to journalArticle

Gion, M, Tremolada, C, Mione, R, Dittadi, R, Palma, DP, Castoro, C, Ruol, A, Nosadini, A, Peracchia, A & Bruscagnin, G 1990, 'Tumor markers in squamous cell carcinoma of esophagus: Immunometric assay in cytosol and membrane fraction', International Journal of Biological Markers, vol. 5, no. 1, pp. 7-13.
Gion, M. ; Tremolada, C. ; Mione, R. ; Dittadi, R. ; Palma, D. P. ; Castoro, C. ; Ruol, A. ; Nosadini, A. ; Peracchia, A. ; Bruscagnin, G. / Tumor markers in squamous cell carcinoma of esophagus : Immunometric assay in cytosol and membrane fraction. In: International Journal of Biological Markers. 1990 ; Vol. 5, No. 1. pp. 7-13.
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abstract = "Carcinoembryonic antigen (CEA), tissue polypeptide antigen (TPA), ferritin, and the monoclonal antibody-detected tumor-associated antigens CA19.9 and CA50 were measured by radioimmunoassay in tissue fractions of carcinoma and normal esophageal mucosa from 59 patients with untreated primary squamous cell carcinoma of the esophagus. Tumor markers were measured in cytosol (118 samples) and in a membrane-enriched fraction (32 samples). CEA, TPA and ferritin were detected in almost all the cytosol samples evaluated, CA19.9 and CA50 in 66{\%} and 50{\%} of cases respectively. Ferritin was significantly higher in carcinoma than in normal mucosa. The cytosol concentrations of CEA, TPA, CA19.9 and CA50 were not significantly different in carcinoma and normal tissue. Concentrations of CEA, CA19.9 and CA50 in the membrane fraction tended to be higher in normal tissue than in carcinoma whereas the cytosol-to-membrane ratio was significantly higher in carcinoma. For CEA, CA19.9 and CA50, the phenotypic pattern of the malignant transformation seems to involve a different intracellular distribution rather than a quantitative change. No correlations were found between tissue and serum concentrations of the tumor markers, the former being related to the phenotypic characteristics of the tumor, the latter to the tumor burden.",
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AU - Mione, R.

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AU - Ruol, A.

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