Tumor microvasculature observed using different contrast agents: A comparison between Gd-DTPA-Albumin and B-22956/1 in an experimental model of mammary carcinoma

Federico Boschi, Pasquina Marzola, Marco Sandri, Elena Nicolato, Mirco Galiè, Silvia Fiorini, Flavia Merigo, Vito Lorusso, Linda Chaabane, Andrea Sbarbati

Research output: Contribution to journalArticle

Abstract

Objective: The aim of this study was to compare a pure macromolecular contrast agent (Gd-DTPA-albumin) with a new protein-binding blood pool contrast agent (B22956/1) in terms of their capacity to investigate the microvasculature in an experimental model of mammary carcinoma. Materials and methods: Tumors were induced by subcutaneous injection of 5 × 10 5 BB1 cells into the backs of 5-7 week-old female FVB/neuNT233 mice. The animals were observed using DCE-MRI when the longest diameter of the tumor was 10.2±2.0 mm. DCE-MRI experiments were carried out using B22956/1 and (24 h later) Gd-DTPA-albumin. Results: DCE-MRI data showed that vasculature in the tumor rim was characterized by greater fractional plasma volume and transendothelial permeability than vasculature in the tumor core as measured by both contrast agents. Permeability to Gd-DTPA-albumin in the tumor core was hardly measurable while permeability to B22956/1 was substantial. Histologically the tumor core showed areas of well vascularized, viable tissue surrounded by necrotic regions. Conclusions: DCE-MRI experiments performed with B22956/1 are useful in the investigation of vasculature in those tumor regions that are characterized by low permeability to macromolecules.

Original languageEnglish
Pages (from-to)169-176
Number of pages8
JournalMagnetic Resonance Materials in Physics, Biology, and Medicine
Volume21
Issue number3
DOIs
Publication statusPublished - May 2008

Keywords

  • Angiogenesis
  • B22956/1
  • Contrast agents
  • DCE-MRI

ASJC Scopus subject areas

  • Biophysics
  • Genetics

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