Tumor microvessel density and prognosis in node-negative breast cancer

Laura Medri, Oriana Nanni, Annalisa Volpi, Emanuela Scarpi, Alessandra Dubini, Angela Riccobon, Aldo Becciolini, Simonetta Bianchi, Dino Amadori

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Microvessel density (MVD) of breast cancer is widely regarded as a prognostic factor, but results from studies on the most important case series have produced conflicting results. The present study was performed with confirmatory intent to define the prognostic relevance of MVD on a series of 378 node-negative-breast-cancer patients, much larger than any other series previously analyzed. Microvessels were stained using Factor-VIII antibody and an immunoperoxidase reaction. MVD was determined independently by 2 observers according to Weidner's methods. In parallel, cell proliferation was evaluated as S-phase fraction and determined according to the 3H-thymidine-labeling index method (TLI). Estrogen and progesterone receptors were quantitatively assessed using the dextran-charcoal technique. Tumor MVD varied greatly from tumor to tumor (2 to 232 MV/mm2) and was unrelated to patient age and menopausal status, or to tumor size, histology and steroid-receptor status. A significant (p = 0.004) but weak inverse correlation (r(s) = -0.188) was observed with cell proliferation. Univariate analysis using 40 MV/mm2 as cut-off showed an inverse relation with 5-year relapse-free survival (82% vs. 71%, p = 0.018). This finding was limited to very small tumors, slowly proliferating tumors and ER-negative tumors. Multivariate analysis identified tumor size and TLI, but not MVD, menopausal status or ER as independent prognostic factors. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish
Pages (from-to)74-80
Number of pages7
JournalInternational Journal of Cancer
Volume89
Issue number1
DOIs
Publication statusPublished - Jan 20 2000

Fingerprint

Microvessels
Breast Neoplasms
Neoplasms
Thymidine
Cell Proliferation
Steroid Receptors
Charcoal
Factor VIII
Progesterone Receptors
Dextrans
S Phase
Estrogen Receptors
Histology
Multivariate Analysis
Recurrence
Survival
Antibodies

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Tumor microvessel density and prognosis in node-negative breast cancer. / Medri, Laura; Nanni, Oriana; Volpi, Annalisa; Scarpi, Emanuela; Dubini, Alessandra; Riccobon, Angela; Becciolini, Aldo; Bianchi, Simonetta; Amadori, Dino.

In: International Journal of Cancer, Vol. 89, No. 1, 20.01.2000, p. 74-80.

Research output: Contribution to journalArticle

Medri, Laura ; Nanni, Oriana ; Volpi, Annalisa ; Scarpi, Emanuela ; Dubini, Alessandra ; Riccobon, Angela ; Becciolini, Aldo ; Bianchi, Simonetta ; Amadori, Dino. / Tumor microvessel density and prognosis in node-negative breast cancer. In: International Journal of Cancer. 2000 ; Vol. 89, No. 1. pp. 74-80.
@article{2bdff0297bc24c7dba0bcb16c61b306f,
title = "Tumor microvessel density and prognosis in node-negative breast cancer",
abstract = "Microvessel density (MVD) of breast cancer is widely regarded as a prognostic factor, but results from studies on the most important case series have produced conflicting results. The present study was performed with confirmatory intent to define the prognostic relevance of MVD on a series of 378 node-negative-breast-cancer patients, much larger than any other series previously analyzed. Microvessels were stained using Factor-VIII antibody and an immunoperoxidase reaction. MVD was determined independently by 2 observers according to Weidner's methods. In parallel, cell proliferation was evaluated as S-phase fraction and determined according to the 3H-thymidine-labeling index method (TLI). Estrogen and progesterone receptors were quantitatively assessed using the dextran-charcoal technique. Tumor MVD varied greatly from tumor to tumor (2 to 232 MV/mm2) and was unrelated to patient age and menopausal status, or to tumor size, histology and steroid-receptor status. A significant (p = 0.004) but weak inverse correlation (r(s) = -0.188) was observed with cell proliferation. Univariate analysis using 40 MV/mm2 as cut-off showed an inverse relation with 5-year relapse-free survival (82{\%} vs. 71{\%}, p = 0.018). This finding was limited to very small tumors, slowly proliferating tumors and ER-negative tumors. Multivariate analysis identified tumor size and TLI, but not MVD, menopausal status or ER as independent prognostic factors. (C) 2000 Wiley-Liss, Inc.",
author = "Laura Medri and Oriana Nanni and Annalisa Volpi and Emanuela Scarpi and Alessandra Dubini and Angela Riccobon and Aldo Becciolini and Simonetta Bianchi and Dino Amadori",
year = "2000",
month = "1",
day = "20",
doi = "10.1002/(SICI)1097-0215(20000120)89:1<74::AID-IJC12>3.0.CO;2-L",
language = "English",
volume = "89",
pages = "74--80",
journal = "International Journal of Cancer",
issn = "0020-7136",
publisher = "Wiley-Liss Inc.",
number = "1",

}

TY - JOUR

T1 - Tumor microvessel density and prognosis in node-negative breast cancer

AU - Medri, Laura

AU - Nanni, Oriana

AU - Volpi, Annalisa

AU - Scarpi, Emanuela

AU - Dubini, Alessandra

AU - Riccobon, Angela

AU - Becciolini, Aldo

AU - Bianchi, Simonetta

AU - Amadori, Dino

PY - 2000/1/20

Y1 - 2000/1/20

N2 - Microvessel density (MVD) of breast cancer is widely regarded as a prognostic factor, but results from studies on the most important case series have produced conflicting results. The present study was performed with confirmatory intent to define the prognostic relevance of MVD on a series of 378 node-negative-breast-cancer patients, much larger than any other series previously analyzed. Microvessels were stained using Factor-VIII antibody and an immunoperoxidase reaction. MVD was determined independently by 2 observers according to Weidner's methods. In parallel, cell proliferation was evaluated as S-phase fraction and determined according to the 3H-thymidine-labeling index method (TLI). Estrogen and progesterone receptors were quantitatively assessed using the dextran-charcoal technique. Tumor MVD varied greatly from tumor to tumor (2 to 232 MV/mm2) and was unrelated to patient age and menopausal status, or to tumor size, histology and steroid-receptor status. A significant (p = 0.004) but weak inverse correlation (r(s) = -0.188) was observed with cell proliferation. Univariate analysis using 40 MV/mm2 as cut-off showed an inverse relation with 5-year relapse-free survival (82% vs. 71%, p = 0.018). This finding was limited to very small tumors, slowly proliferating tumors and ER-negative tumors. Multivariate analysis identified tumor size and TLI, but not MVD, menopausal status or ER as independent prognostic factors. (C) 2000 Wiley-Liss, Inc.

AB - Microvessel density (MVD) of breast cancer is widely regarded as a prognostic factor, but results from studies on the most important case series have produced conflicting results. The present study was performed with confirmatory intent to define the prognostic relevance of MVD on a series of 378 node-negative-breast-cancer patients, much larger than any other series previously analyzed. Microvessels were stained using Factor-VIII antibody and an immunoperoxidase reaction. MVD was determined independently by 2 observers according to Weidner's methods. In parallel, cell proliferation was evaluated as S-phase fraction and determined according to the 3H-thymidine-labeling index method (TLI). Estrogen and progesterone receptors were quantitatively assessed using the dextran-charcoal technique. Tumor MVD varied greatly from tumor to tumor (2 to 232 MV/mm2) and was unrelated to patient age and menopausal status, or to tumor size, histology and steroid-receptor status. A significant (p = 0.004) but weak inverse correlation (r(s) = -0.188) was observed with cell proliferation. Univariate analysis using 40 MV/mm2 as cut-off showed an inverse relation with 5-year relapse-free survival (82% vs. 71%, p = 0.018). This finding was limited to very small tumors, slowly proliferating tumors and ER-negative tumors. Multivariate analysis identified tumor size and TLI, but not MVD, menopausal status or ER as independent prognostic factors. (C) 2000 Wiley-Liss, Inc.

UR - http://www.scopus.com/inward/record.url?scp=0034688124&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034688124&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1097-0215(20000120)89:1<74::AID-IJC12>3.0.CO;2-L

DO - 10.1002/(SICI)1097-0215(20000120)89:1<74::AID-IJC12>3.0.CO;2-L

M3 - Article

C2 - 10719734

AN - SCOPUS:0034688124

VL - 89

SP - 74

EP - 80

JO - International Journal of Cancer

JF - International Journal of Cancer

SN - 0020-7136

IS - 1

ER -