Tumor necrosis factor-α and interleukin-6 gene polymorphism association with susceptibility to celiac disease in italian patients

R. M. de Albuquerque Maranhão, F. A. Martins Esteves, S. Crovella, L. Segat, P. R. Eleutério Souza

Research output: Contribution to journalArticlepeer-review

Abstract

The aim of this research was to study polymorphisms in the genes encoding cytokines interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) in patients with celiac disease (CD) antigens DQ2 (DQ2-positive) or DQ8 (DQ8-positive). We compared the results with healthy controls to determine whether any of the polymorphisms have a role in susceptibility to CD. A case-control of 192 patients with CD (96 DQ2-positive and 96 DQ8-positive) and 96 healthy controls from northeast Italy were included in the study. Analysis of single nucleotide polymorphisms (SNPs) was carried out using the polymerase chain reaction-restriction fragment length polymorphism method. Significant differences for the TNF-α (-308 G>A) polymorphism were observed when we compared the flowing groups: DQ2-positive with controls [odds ratio (OR) = 0.45, P = 0.0002]; DQ8-positive with controls (OR = 3.55, P <0.0001); and DQ2-positive with DQ8-positive (OR = 0.12, P <0.0001). We did not observe a statistically significant association between IL-6 (-174 G>C) polymorphism and CD (P > 0.05). Our results suggest that TNF-α (-308 G>A) polymorphism may play a role in susceptibility to CD in Italian patients.

Original languageEnglish
Pages (from-to)16343-16352
Number of pages10
JournalGenetics and Molecular Research
Volume14
Issue number4
DOIs
Publication statusPublished - Dec 9 2015

Keywords

  • Celiac disease
  • HLA-DQ
  • IL-6
  • TNF-α

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Fingerprint Dive into the research topics of 'Tumor necrosis factor-α and interleukin-6 gene polymorphism association with susceptibility to celiac disease in italian patients'. Together they form a unique fingerprint.

Cite this