TY - JOUR
T1 - Tumor necrosis factor-α and interleukin-6 gene polymorphism association with susceptibility to celiac disease in italian patients
AU - de Albuquerque Maranhão, R. M.
AU - Martins Esteves, F. A.
AU - Crovella, S.
AU - Segat, L.
AU - Eleutério Souza, P. R.
PY - 2015/12/9
Y1 - 2015/12/9
N2 - The aim of this research was to study polymorphisms in the genes encoding cytokines interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) in patients with celiac disease (CD) antigens DQ2 (DQ2-positive) or DQ8 (DQ8-positive). We compared the results with healthy controls to determine whether any of the polymorphisms have a role in susceptibility to CD. A case-control of 192 patients with CD (96 DQ2-positive and 96 DQ8-positive) and 96 healthy controls from northeast Italy were included in the study. Analysis of single nucleotide polymorphisms (SNPs) was carried out using the polymerase chain reaction-restriction fragment length polymorphism method. Significant differences for the TNF-α (-308 G>A) polymorphism were observed when we compared the flowing groups: DQ2-positive with controls [odds ratio (OR) = 0.45, P = 0.0002]; DQ8-positive with controls (OR = 3.55, P <0.0001); and DQ2-positive with DQ8-positive (OR = 0.12, P <0.0001). We did not observe a statistically significant association between IL-6 (-174 G>C) polymorphism and CD (P > 0.05). Our results suggest that TNF-α (-308 G>A) polymorphism may play a role in susceptibility to CD in Italian patients.
AB - The aim of this research was to study polymorphisms in the genes encoding cytokines interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) in patients with celiac disease (CD) antigens DQ2 (DQ2-positive) or DQ8 (DQ8-positive). We compared the results with healthy controls to determine whether any of the polymorphisms have a role in susceptibility to CD. A case-control of 192 patients with CD (96 DQ2-positive and 96 DQ8-positive) and 96 healthy controls from northeast Italy were included in the study. Analysis of single nucleotide polymorphisms (SNPs) was carried out using the polymerase chain reaction-restriction fragment length polymorphism method. Significant differences for the TNF-α (-308 G>A) polymorphism were observed when we compared the flowing groups: DQ2-positive with controls [odds ratio (OR) = 0.45, P = 0.0002]; DQ8-positive with controls (OR = 3.55, P <0.0001); and DQ2-positive with DQ8-positive (OR = 0.12, P <0.0001). We did not observe a statistically significant association between IL-6 (-174 G>C) polymorphism and CD (P > 0.05). Our results suggest that TNF-α (-308 G>A) polymorphism may play a role in susceptibility to CD in Italian patients.
KW - Celiac disease
KW - HLA-DQ
KW - IL-6
KW - TNF-α
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U2 - 10.4238/2015.December.9.2
DO - 10.4238/2015.December.9.2
M3 - Article
C2 - 26662429
AN - SCOPUS:84949845240
VL - 14
SP - 16343
EP - 16352
JO - Genetics and Molecular Research
JF - Genetics and Molecular Research
SN - 1676-5680
IS - 4
ER -