TY - JOUR
T1 - Tumor necrosis factor α-induced vascular leakage involves PECAM1 phosphorylation
AU - Ferrero, Elisabetta
AU - Villa, Antonello
AU - Ferrero, Maria Elena
AU - Toninelli, Elisabetta
AU - Bender, Jeffrey R.
AU - Pardi, Ruggero
AU - Zocchi, Maria Raffaella
PY - 1996/7/15
Y1 - 1996/7/15
N2 - Herein we show that exposure of human umbilical vein endothelial cells to tumor necrosis factor α (TNFα) led to platelet endothelial cell adhesion molecule-1 (PECAM1) surface redistribution, disruption of cytoskeleton connections, and increased PECAM1 phosphorylation, accompanied by increased permeability to macromolecules. The in vitro use of inhibitors of tyrosine or serine-threonine kinases could prevent both PECAM1 surface redistribution and the increase in permeability induced by the cytokine. In vivo administration of lavendustin A, a natural tyrosine kinase inhibitor, protected endothelial cells from TNFα-dependent vascular leakage in mouse liver. We propose that the involvement of PECAM1 in TNFα-mediated effects on vascular permeability may depend on a dynamically regulated cytoskeletal association, related to the degree of PECAM1 phosphorylation.
AB - Herein we show that exposure of human umbilical vein endothelial cells to tumor necrosis factor α (TNFα) led to platelet endothelial cell adhesion molecule-1 (PECAM1) surface redistribution, disruption of cytoskeleton connections, and increased PECAM1 phosphorylation, accompanied by increased permeability to macromolecules. The in vitro use of inhibitors of tyrosine or serine-threonine kinases could prevent both PECAM1 surface redistribution and the increase in permeability induced by the cytokine. In vivo administration of lavendustin A, a natural tyrosine kinase inhibitor, protected endothelial cells from TNFα-dependent vascular leakage in mouse liver. We propose that the involvement of PECAM1 in TNFα-mediated effects on vascular permeability may depend on a dynamically regulated cytoskeletal association, related to the degree of PECAM1 phosphorylation.
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M3 - Article
C2 - 8764109
AN - SCOPUS:0029982830
VL - 56
SP - 3211
EP - 3215
JO - Journal of Cancer Research
JF - Journal of Cancer Research
SN - 0008-5472
IS - 14
ER -