A progressive increase of cell proliferation occurs with the progression of H. pylori induced mucosal damage until the development of IM. Nevertheless, epithelial apoptosis does not accompany this phenomenon with a parallel pattern since apoptotic index shows the tendency to an initial rise after infection, followed by a decrease with the progression towards IM. Moreover, H. pylori related Th1 flogosis is strongly mediated by TNFa mucosal release; a recent evidence suggests its involvement in the modulation of epithelial apoptosis. On these bases, a recent experience of ours concerns a study performed with the aim of evaluating simultaneously, in the progressive stages of H. pylon related gastric lesions, the following events: proliferationlopoptosis of gastric epithelium and TNFα mucosal release. Antral biopsies from 20 H. pylori + patients were studied: 10 with (IM group) and 10 without (CG group) foci of IM; 10 antral samples ofdyspeptics with normal H. pylon - stomach (N) were used as control. The following parameters were evaluated by immunohistochemistyy: proliferating cell nuclear antigen (PCNA) labelling index (LI) for epithelial proliferation, 85 kDa caspase-cleaved fragment (p85) of human poly (ADP-ribose) polymerase (PARP) LI for epithelial apoptosis and TNFα LI in stromal cells. One way Analysis of variance (ANOVA) plus Student-Neumann-Keuls tests were used for statistical analysis. Our results showed: PCNA LI: IM > CG > N (p <0.001); PARP LI: CG > IM > N (p <0.05) and TNFα LI: CG > IM > N (p <0.001). Our data confirm that IM is charactenised by a lowered rate of apoptosis along with a marked raised epithelial regeneration, thus suggesting a lack of the balance of cellular turnover, which strongly supports the concept of this lesion as a malignant precursor. The decreased release of TNFα, we have found in areas of IM, is probably due to the disappearance of H. pylori from this site and could be relevant to effect the increased ratio proliferation/death of gastric epithelium.
|Translated title of the contribution||Tumor necrosis factor alpha (TNFα) in regulating the proliferation and death of epithelial cells in Helicobacter pylori-correlated gastric lesions|
|Number of pages||3|
|Journal||Argomenti di Gastroenterologia Clinica|
|Publication status||Published - Mar 2002|
ASJC Scopus subject areas