Abstract
Treatment of human umbilical cord vein endothelial cells with tumor necrosis factor results in marked changes in cell shape and cytoskeletal organization. After 4 h of treatment, these cells loose reciprocal contacts with the formation of intercellular gaps. This retraction reaches a maximum after 6 h when most stress fibers staining for F-actin disappear and vinculin becomes diffused in the cytoplasm. Such changes spontaneously reverse after 24 h in the presence of tumor necrosis factor or after 2 h of incubation in fresh medium. After treatment with tumor necrosis factor, endothelial monolayers become permeable to albumin because of gaps that form between cells. Normal human serum, plasma ±-proteinase inhibitor and an anti-inflammatory peptide that decrease synthesis of platelet-activating factor inhibit the changes induced by tumor necrosis factor. Furthermore, receptor antagonists of platelet-activating factor have the same effect.These findings suggest that platelet-activating factor is a secondary mediator responsible for the changes in ceil shapeand cytoskeletal organization, and for the leakiness of endothelial monolayers.
Original language | English |
---|---|
Pages (from-to) | 84-91 |
Number of pages | 8 |
Journal | International Archives of Allergy and Immunology |
Volume | 96 |
Issue number | 1 |
DOIs | |
Publication status | Published - 1991 |
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ASJC Scopus subject areas
- Immunology
- Immunology and Allergy
Cite this
Tumor necrosis factor alters cytoskeletal organization and barrier function of endothelial cells. / Camussi, G.; Turello, E.; Bussolinoc, F.; Baglioni, Corrado.
In: International Archives of Allergy and Immunology, Vol. 96, No. 1, 1991, p. 84-91.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Tumor necrosis factor alters cytoskeletal organization and barrier function of endothelial cells
AU - Camussi, G.
AU - Turello, E.
AU - Bussolinoc, F.
AU - Baglioni, Corrado
PY - 1991
Y1 - 1991
N2 - Treatment of human umbilical cord vein endothelial cells with tumor necrosis factor results in marked changes in cell shape and cytoskeletal organization. After 4 h of treatment, these cells loose reciprocal contacts with the formation of intercellular gaps. This retraction reaches a maximum after 6 h when most stress fibers staining for F-actin disappear and vinculin becomes diffused in the cytoplasm. Such changes spontaneously reverse after 24 h in the presence of tumor necrosis factor or after 2 h of incubation in fresh medium. After treatment with tumor necrosis factor, endothelial monolayers become permeable to albumin because of gaps that form between cells. Normal human serum, plasma ±-proteinase inhibitor and an anti-inflammatory peptide that decrease synthesis of platelet-activating factor inhibit the changes induced by tumor necrosis factor. Furthermore, receptor antagonists of platelet-activating factor have the same effect.These findings suggest that platelet-activating factor is a secondary mediator responsible for the changes in ceil shapeand cytoskeletal organization, and for the leakiness of endothelial monolayers.
AB - Treatment of human umbilical cord vein endothelial cells with tumor necrosis factor results in marked changes in cell shape and cytoskeletal organization. After 4 h of treatment, these cells loose reciprocal contacts with the formation of intercellular gaps. This retraction reaches a maximum after 6 h when most stress fibers staining for F-actin disappear and vinculin becomes diffused in the cytoplasm. Such changes spontaneously reverse after 24 h in the presence of tumor necrosis factor or after 2 h of incubation in fresh medium. After treatment with tumor necrosis factor, endothelial monolayers become permeable to albumin because of gaps that form between cells. Normal human serum, plasma ±-proteinase inhibitor and an anti-inflammatory peptide that decrease synthesis of platelet-activating factor inhibit the changes induced by tumor necrosis factor. Furthermore, receptor antagonists of platelet-activating factor have the same effect.These findings suggest that platelet-activating factor is a secondary mediator responsible for the changes in ceil shapeand cytoskeletal organization, and for the leakiness of endothelial monolayers.
UR - http://www.scopus.com/inward/record.url?scp=0025997208&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025997208&partnerID=8YFLogxK
U2 - 10.1159/000235539
DO - 10.1159/000235539
M3 - Article
C2 - 1752699
AN - SCOPUS:0025997208
VL - 96
SP - 84
EP - 91
JO - International Archives of Allergy and Immunology
JF - International Archives of Allergy and Immunology
SN - 1018-2438
IS - 1
ER -