Tumor necrosis factor alters cytoskeletal organization and barrier function of endothelial cells

G. Camussi, E. Turello, F. Bussolinoc, Corrado Baglioni

Research output: Contribution to journalArticle

Abstract

Treatment of human umbilical cord vein endothelial cells with tumor necrosis factor results in marked changes in cell shape and cytoskeletal organization. After 4 h of treatment, these cells loose reciprocal contacts with the formation of intercellular gaps. This retraction reaches a maximum after 6 h when most stress fibers staining for F-actin disappear and vinculin becomes diffused in the cytoplasm. Such changes spontaneously reverse after 24 h in the presence of tumor necrosis factor or after 2 h of incubation in fresh medium. After treatment with tumor necrosis factor, endothelial monolayers become permeable to albumin because of gaps that form between cells. Normal human serum, plasma ±-proteinase inhibitor and an anti-inflammatory peptide that decrease synthesis of platelet-activating factor inhibit the changes induced by tumor necrosis factor. Furthermore, receptor antagonists of platelet-activating factor have the same effect.These findings suggest that platelet-activating factor is a secondary mediator responsible for the changes in ceil shapeand cytoskeletal organization, and for the leakiness of endothelial monolayers.

Original languageEnglish
Pages (from-to)84-91
Number of pages8
JournalInternational Archives of Allergy and Immunology
Volume96
Issue number1
DOIs
Publication statusPublished - 1991

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

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