Tumor-necrosis-factor-induced fibroblast growth factor-1 acts as a survival factor in a transformed endothelial cell line

J. A M Maier, Daniele Morelli, Sylvie Ménard, Maria I. Colnaghi, Andrea Balsari

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Endothelial cells undergo apoptosis after withdrawal of growth factors, alterations in the extracellular matrix, or exposure to cytokines. Here we report that tumor necrosis factor (TNF)-α induces apoptosis of human endothelial cells derived from the umbilical vein in a dose-dependent fashion. Apoptosis is triggered through a pathway that is independent from the levels of Bcl-2. On the contrary, TNF stimulates the growth of spontaneously transformed human umbilical vein endothelial cells. This proliferative effect is mediated through the up-regulation of fibroblast growth factor-1 by TNF. The addition of specific fibroblast growth factor-1 antisense oligonucleotides inhibits TNF-induced fibroblast growth factor-1 expression, thus inhibiting the growth and triggering apoptosis of spontaneously, transformed human umbilical vein endothelial cells.

Original languageEnglish
Pages (from-to)945-952
Number of pages8
JournalAmerican Journal of Pathology
Volume149
Issue number3
Publication statusPublished - Sep 1996

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Fibroblast Growth Factor 1
Transformed Cell Line
Human Umbilical Vein Endothelial Cells
Endothelial Cells
Tumor Necrosis Factor-alpha
Apoptosis
Antisense Oligonucleotides
Growth
Extracellular Matrix
Intercellular Signaling Peptides and Proteins
Up-Regulation
Cytokines

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Tumor-necrosis-factor-induced fibroblast growth factor-1 acts as a survival factor in a transformed endothelial cell line. / Maier, J. A M; Morelli, Daniele; Ménard, Sylvie; Colnaghi, Maria I.; Balsari, Andrea.

In: American Journal of Pathology, Vol. 149, No. 3, 09.1996, p. 945-952.

Research output: Contribution to journalArticle

Maier, J. A M ; Morelli, Daniele ; Ménard, Sylvie ; Colnaghi, Maria I. ; Balsari, Andrea. / Tumor-necrosis-factor-induced fibroblast growth factor-1 acts as a survival factor in a transformed endothelial cell line. In: American Journal of Pathology. 1996 ; Vol. 149, No. 3. pp. 945-952.
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