Tumor necrosis factor-related apoptosis-inducing ligand promotes migration of human bone marrow multipotent stromal cells

Paola Secchiero, Elisabetta Melloni, Federica Corallini, Antonio Paolo Beltrami, Francesco Alviano, Daniela Milani, Federica D'Aurizio, Maria Grazia Di Iasio, Daniela Cesselli, Gian Paolo Bagnara, Giorgio Zauli

Research output: Contribution to journalArticle

Abstract

Adult multipotent stromal cells (MSCs), also known as mesenchymal stem cells, represent an important source of cells for the repair of a number of damaged tissues. Both bone marrow (BM)-derived and amniotic MSCs expressed detectable surface levels of two (tumor necrosis factor-related apoptosis-inducing ligand receptor 2 [TRAIL-R2] and TRAIL-R4) of four transmembrane TRAIL receptors. Although the best-characterized activity of TRAIL-R2 is the transduction of apoptotic signals, neither recombinant TRAIL (rTRAIL) nor infection with an adenovirus-expressing TRAIL induced cytotoxic effects on MSCs. Moreover, whereas rTRAIL did not affect proliferation or differentiation of MSCs along the osteogenic and adipogenic lineages, it significantly promoted the migration of human MSCs in range of concentrations comparable to that of soluble TRAIL in human plasma (100 pg/ml). Since rTRAIL induced the rapid phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2) in MSC cultures and pretreatment with pharmacological inhibitors of the ERK1/2 pathway efficiently counteracted the rTRAIL-induced human MSC migration, these data indicate that ERK1/2 is involved in mediating the ability of rTRAIL to stimulate MSC migration. Taking into consideration that the soluble factors able to induce MSC migration have not been extensively characterized, our current data indicate that the TRAIL/TRAIL-R system might play an important role in the biology of MSCs.

Original languageEnglish
Pages (from-to)2955-2963
Number of pages9
JournalStem Cells
Volume26
Issue number11
DOIs
Publication statusPublished - Nov 2008

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Stromal Cells
Mesenchymal Stromal Cells
Tumor Necrosis Factor-alpha
Apoptosis
Ligands
Mitogen-Activated Protein Kinase 3
Mitogen-Activated Protein Kinase 1
TNF-Related Apoptosis-Inducing Ligand Receptors
Cell Movement
Adenoviridae Infections
Signal Transduction
Cell Culture Techniques
Bone Marrow
Phosphorylation
Pharmacology

Keywords

  • Mesenchymal stem cells
  • Migration
  • Multipotent precursor
  • Tumor necrosis factor-related apoptosis-inducing ligand
  • Tumor necrosis factor-related apoptosis-inducing ligand receptors

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Molecular Medicine

Cite this

Secchiero, P., Melloni, E., Corallini, F., Beltrami, A. P., Alviano, F., Milani, D., ... Zauli, G. (2008). Tumor necrosis factor-related apoptosis-inducing ligand promotes migration of human bone marrow multipotent stromal cells. Stem Cells, 26(11), 2955-2963. https://doi.org/10.1634/stemcells.2008-0512

Tumor necrosis factor-related apoptosis-inducing ligand promotes migration of human bone marrow multipotent stromal cells. / Secchiero, Paola; Melloni, Elisabetta; Corallini, Federica; Beltrami, Antonio Paolo; Alviano, Francesco; Milani, Daniela; D'Aurizio, Federica; Di Iasio, Maria Grazia; Cesselli, Daniela; Bagnara, Gian Paolo; Zauli, Giorgio.

In: Stem Cells, Vol. 26, No. 11, 11.2008, p. 2955-2963.

Research output: Contribution to journalArticle

Secchiero, P, Melloni, E, Corallini, F, Beltrami, AP, Alviano, F, Milani, D, D'Aurizio, F, Di Iasio, MG, Cesselli, D, Bagnara, GP & Zauli, G 2008, 'Tumor necrosis factor-related apoptosis-inducing ligand promotes migration of human bone marrow multipotent stromal cells', Stem Cells, vol. 26, no. 11, pp. 2955-2963. https://doi.org/10.1634/stemcells.2008-0512
Secchiero, Paola ; Melloni, Elisabetta ; Corallini, Federica ; Beltrami, Antonio Paolo ; Alviano, Francesco ; Milani, Daniela ; D'Aurizio, Federica ; Di Iasio, Maria Grazia ; Cesselli, Daniela ; Bagnara, Gian Paolo ; Zauli, Giorgio. / Tumor necrosis factor-related apoptosis-inducing ligand promotes migration of human bone marrow multipotent stromal cells. In: Stem Cells. 2008 ; Vol. 26, No. 11. pp. 2955-2963.
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