Tumor promoter phorbol myristate acetate inhibits Ca2+ influx through voltage-gated Ca2+ channels in two secretory cell lines, PC12 and RINm5F

F. Di Virgilio, T. Pozzan, C. B. Wollheim, L. M. Vicentini, J. Meldolesi

Research output: Contribution to journalArticlepeer-review

Abstract

Protein kinase C is known to be involved both in initiation and termination of cellular responses due to phosphoinositide breakdown. Here we report that in PC12 cells (a line of neurosecretory cells derived from a rat pheochromocytoma), pretreatment with nanomolar concentrations of phorbol myristate acetate, PMA, which is believed to specifically activate protein kinase C, inhibits the cytosolic-free Ca2+ concentration rise induced by depolarizing agents. In contrast, plasma membrane potential and 45Ca efflux from preloaded cells were unaffected by PMA pretreatment. Inhibition by PMA and diacylglycerol of the cytosolic-free Ca2+ concentration rise induced by depolarization was observed also in another cell line, the insulin secreting line RINm5F. These results raise the possibility that the voltage-gated Ca2+ channel is under inhibitory control by protein kinase C.

Original languageEnglish
Pages (from-to)32-35
Number of pages4
JournalJournal of Biological Chemistry
Volume261
Issue number1
Publication statusPublished - 1986

ASJC Scopus subject areas

  • Biochemistry

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