Tumor promoter phorbol myristate acetate inhibits Ca2+ influx through voltage-gated Ca2+ channels in two secretory cell lines, PC12 and RINm5F

F. Di Virgilio, T. Pozzan, C. B. Wollheim, L. M. Vicentini, J. Meldolesi

Research output: Contribution to journalArticle

Abstract

Protein kinase C is known to be involved both in initiation and termination of cellular responses due to phosphoinositide breakdown. Here we report that in PC12 cells (a line of neurosecretory cells derived from a rat pheochromocytoma), pretreatment with nanomolar concentrations of phorbol myristate acetate, PMA, which is believed to specifically activate protein kinase C, inhibits the cytosolic-free Ca2+ concentration rise induced by depolarizing agents. In contrast, plasma membrane potential and 45Ca efflux from preloaded cells were unaffected by PMA pretreatment. Inhibition by PMA and diacylglycerol of the cytosolic-free Ca2+ concentration rise induced by depolarization was observed also in another cell line, the insulin secreting line RINm5F. These results raise the possibility that the voltage-gated Ca2+ channel is under inhibitory control by protein kinase C.

Original languageEnglish
Pages (from-to)32-35
Number of pages4
JournalJournal of Biological Chemistry
Volume261
Issue number1
Publication statusPublished - 1986

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Tetradecanoylphorbol Acetate
Carcinogens
Protein Kinase C
Cells
Cell Line
Electric potential
PC12 Cells
Diglycerides
Depolarization
Pheochromocytoma
Cell membranes
Phosphatidylinositols
Membrane Potentials
Rats
Cell Membrane
Insulin

ASJC Scopus subject areas

  • Biochemistry

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Tumor promoter phorbol myristate acetate inhibits Ca2+ influx through voltage-gated Ca2+ channels in two secretory cell lines, PC12 and RINm5F. / Di Virgilio, F.; Pozzan, T.; Wollheim, C. B.; Vicentini, L. M.; Meldolesi, J.

In: Journal of Biological Chemistry, Vol. 261, No. 1, 1986, p. 32-35.

Research output: Contribution to journalArticle

Di Virgilio, F. ; Pozzan, T. ; Wollheim, C. B. ; Vicentini, L. M. ; Meldolesi, J. / Tumor promoter phorbol myristate acetate inhibits Ca2+ influx through voltage-gated Ca2+ channels in two secretory cell lines, PC12 and RINm5F. In: Journal of Biological Chemistry. 1986 ; Vol. 261, No. 1. pp. 32-35.
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