Tumor protein D52 (TPD52): A novel B-cell/plasma-cell molecule with unique expression pattern and Ca2+-dependent association with annexin VI

Enrico Tiacci, Pier Luigi Orvietani, Barbara Bigerna, Alessandra Pucciarini, Garry L. Corthals, Valentina Pettirossi, Maria P. Martelli, Arcangelo Liso, Roberta Benedetti, Roberta Pacini, Niccolò Bolli, Stefano Pileri, Karen Pulford, Marcello Gambacorta, Antonino Carbone, Carla Pasquarello, Alexander Scherl, Helen Robertson, Maria Teresa Sciurpi, Giovanni Alunni-BistocchiLuciano Binaglia, Jennifer A. Byrne, Brunangelo Falini

Research output: Contribution to journalArticle

Abstract

We generated a murine monoclonal antibody (B28p) detecting an antigenic determinant shared by the immunoglobulin superfamily receptor translocation-associated 1 (IRTA1) receptor (the immunogen used to raise B28p) and an unrelated 28-kDa protein that was subsequently subjected to extensive characterization. The expression of the 28-kDa protein in normal lymphohematopoietic tissues was restricted to B cells and plasma cells and clearly differed from that expected for IRTA1 (selectively expressed by mucosa-associated lymphoid tissue [MALT] marginal zone B cells). Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE)/mass-spectrometry analysis identified the 28-kDa protein as human tumor protein D52 (TPD52), whose expression had been previously described only in normal and neoplastic epithelia. Specific B28p reactivity with TPD52 was confirmed by immunostaining/immunoblotting of TPD52-transfected cells. TPD52 expression pattern in normal and neoplastic B cells was unique. In fact, unlike other B-cell molecules (paired box 5 [PAX5], CD19, CD79a, CD20, CD22), which are down-regulated during differentiation from B cells to plasma cells, TPD52 expression reached its maximum levels at the plasma cell stage. In the Thiel myeloma cell line, TPD52 bound to annexin VI in a Ca2+-dependent manner, suggesting that these molecules may act in concert to regulate secretory processes in plasma cells, similarly to what was observed in pancreatic acinar cells. Finally, the anti-TPD52 monoclonal antibody served as a valuable tool for the diagnosis of B-cell malignancies.

Original languageEnglish
Pages (from-to)2812-2820
Number of pages9
JournalBlood
Volume105
Issue number7
DOIs
Publication statusPublished - Apr 1 2005

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Annexin A6
Plasma Cells
Tumors
B-Lymphocytes
Cells
Association reactions
Plasmas
Molecules
Neoplasms
Proteins
Electrophoresis, Gel, Two-Dimensional
Immunoglobulins
Monoclonal Antibodies
Tissue
Plasmacytoma
Acinar Cells
Secretory Pathway
Lymphoid Tissue
Electrophoresis
Immunoblotting

ASJC Scopus subject areas

  • Hematology

Cite this

Tiacci, E., Orvietani, P. L., Bigerna, B., Pucciarini, A., Corthals, G. L., Pettirossi, V., ... Falini, B. (2005). Tumor protein D52 (TPD52): A novel B-cell/plasma-cell molecule with unique expression pattern and Ca2+-dependent association with annexin VI. Blood, 105(7), 2812-2820. https://doi.org/10.1182/blood-2004-07-2630

Tumor protein D52 (TPD52) : A novel B-cell/plasma-cell molecule with unique expression pattern and Ca2+-dependent association with annexin VI. / Tiacci, Enrico; Orvietani, Pier Luigi; Bigerna, Barbara; Pucciarini, Alessandra; Corthals, Garry L.; Pettirossi, Valentina; Martelli, Maria P.; Liso, Arcangelo; Benedetti, Roberta; Pacini, Roberta; Bolli, Niccolò; Pileri, Stefano; Pulford, Karen; Gambacorta, Marcello; Carbone, Antonino; Pasquarello, Carla; Scherl, Alexander; Robertson, Helen; Sciurpi, Maria Teresa; Alunni-Bistocchi, Giovanni; Binaglia, Luciano; Byrne, Jennifer A.; Falini, Brunangelo.

In: Blood, Vol. 105, No. 7, 01.04.2005, p. 2812-2820.

Research output: Contribution to journalArticle

Tiacci, E, Orvietani, PL, Bigerna, B, Pucciarini, A, Corthals, GL, Pettirossi, V, Martelli, MP, Liso, A, Benedetti, R, Pacini, R, Bolli, N, Pileri, S, Pulford, K, Gambacorta, M, Carbone, A, Pasquarello, C, Scherl, A, Robertson, H, Sciurpi, MT, Alunni-Bistocchi, G, Binaglia, L, Byrne, JA & Falini, B 2005, 'Tumor protein D52 (TPD52): A novel B-cell/plasma-cell molecule with unique expression pattern and Ca2+-dependent association with annexin VI', Blood, vol. 105, no. 7, pp. 2812-2820. https://doi.org/10.1182/blood-2004-07-2630
Tiacci, Enrico ; Orvietani, Pier Luigi ; Bigerna, Barbara ; Pucciarini, Alessandra ; Corthals, Garry L. ; Pettirossi, Valentina ; Martelli, Maria P. ; Liso, Arcangelo ; Benedetti, Roberta ; Pacini, Roberta ; Bolli, Niccolò ; Pileri, Stefano ; Pulford, Karen ; Gambacorta, Marcello ; Carbone, Antonino ; Pasquarello, Carla ; Scherl, Alexander ; Robertson, Helen ; Sciurpi, Maria Teresa ; Alunni-Bistocchi, Giovanni ; Binaglia, Luciano ; Byrne, Jennifer A. ; Falini, Brunangelo. / Tumor protein D52 (TPD52) : A novel B-cell/plasma-cell molecule with unique expression pattern and Ca2+-dependent association with annexin VI. In: Blood. 2005 ; Vol. 105, No. 7. pp. 2812-2820.
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AU - Tiacci, Enrico

AU - Orvietani, Pier Luigi

AU - Bigerna, Barbara

AU - Pucciarini, Alessandra

AU - Corthals, Garry L.

AU - Pettirossi, Valentina

AU - Martelli, Maria P.

AU - Liso, Arcangelo

AU - Benedetti, Roberta

AU - Pacini, Roberta

AU - Bolli, Niccolò

AU - Pileri, Stefano

AU - Pulford, Karen

AU - Gambacorta, Marcello

AU - Carbone, Antonino

AU - Pasquarello, Carla

AU - Scherl, Alexander

AU - Robertson, Helen

AU - Sciurpi, Maria Teresa

AU - Alunni-Bistocchi, Giovanni

AU - Binaglia, Luciano

AU - Byrne, Jennifer A.

AU - Falini, Brunangelo

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