TY - JOUR
T1 - Tumour growth fraction and apoptosis in salivary gland acinic cell carcinomas. Prognostic implications of Ki-67 and bcl-2 expression and of in situ end labelling (tunel)
AU - Hellquist, Henrik B.
AU - Sundelin, Kaarina
AU - Di Bacco, Alessandra
AU - Tytor, Macie
AU - Manzotti, Michela
AU - Viale, Giuseppe
PY - 1997/3
Y1 - 1997/3
N2 - bcl-2 protein and Ki-67 (MIB-1) mere studied in 32 acinic cell carcinomas (ACCs), all with a minimum of 5 years' clinical follow-up. Tumour apoptosis was evaluated by TdT dUTP nick end labelling (TUNEL) and by morphological criteria. Five patients died of their disease. Patients with stage I tumours had significantly better survival compared with other stages (P <0.05). Patients with h?W-l-negative rumours had significantly better survival than patients with MIB-1-positive rumours (P = 0.05). This study confirms a previous report that MIB-1 is an independent prognostic factor for survival in patients with ACC. Stage I tumours had high expression of bcl-2 protein, but there was no difference when compared with other stages. TUNEL positivity was most prevalent in stage I tumours, compared with stages II, III, and IV (P <0.05), probably indicating more apoptosis. This could imply a capacity of stage I tumours ('early tumours') for early selection of tumour cells for elimination by apoptosis. There was no significant difference between expression of bcl-2 and TUNEL, between these parameters and clinical outcome, or between any parameter and morphological subclassification. We conclude that MIB-1 has prognostic value in ACC. Clinical staging, bcl-2, and TUNEL are also potentially useful as prognostic markers.
AB - bcl-2 protein and Ki-67 (MIB-1) mere studied in 32 acinic cell carcinomas (ACCs), all with a minimum of 5 years' clinical follow-up. Tumour apoptosis was evaluated by TdT dUTP nick end labelling (TUNEL) and by morphological criteria. Five patients died of their disease. Patients with stage I tumours had significantly better survival compared with other stages (P <0.05). Patients with h?W-l-negative rumours had significantly better survival than patients with MIB-1-positive rumours (P = 0.05). This study confirms a previous report that MIB-1 is an independent prognostic factor for survival in patients with ACC. Stage I tumours had high expression of bcl-2 protein, but there was no difference when compared with other stages. TUNEL positivity was most prevalent in stage I tumours, compared with stages II, III, and IV (P <0.05), probably indicating more apoptosis. This could imply a capacity of stage I tumours ('early tumours') for early selection of tumour cells for elimination by apoptosis. There was no significant difference between expression of bcl-2 and TUNEL, between these parameters and clinical outcome, or between any parameter and morphological subclassification. We conclude that MIB-1 has prognostic value in ACC. Clinical staging, bcl-2, and TUNEL are also potentially useful as prognostic markers.
KW - Apoptosis
KW - bcl-2
KW - End labelling
KW - Ki-67
KW - Salivary neoplasm
KW - TUNEL
UR - http://www.scopus.com/inward/record.url?scp=0030914632&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030914632&partnerID=8YFLogxK
U2 - 10.1002/(SICI)1096-9896(199703)181:3<323::AID-PATH780>3.0.CO;2-K
DO - 10.1002/(SICI)1096-9896(199703)181:3<323::AID-PATH780>3.0.CO;2-K
M3 - Article
C2 - 9155720
AN - SCOPUS:0030914632
VL - 181
SP - 323
EP - 329
JO - Journal of Pathology
JF - Journal of Pathology
SN - 0022-3417
IS - 3
ER -