Tumour necrosis factor stimulates endothelin-1 gene expression in cultured bovine endothelial cells

Silvia Orisio, Marina Morigi, Carla Zoja, Norberto Perico, Giuseppe Remuzzi, Norberto Perico, Giuseppe Remuzzi

Research output: Contribution to journalArticle

Abstract

We have studied the effect of human recombinant tumour necrosis factor-α (TNF-α) on gene expression and production of endothelin-1 in cultured bovine aortic endothelial cells. TNF-α (10 and 100 ng ml-1) increased in a time dependent manner the preproendothelin-1 mRNA levels in respect to unstimulated endothelial cells. TNF-α induced endothelin-1 gene expression was associated with a parallel increase in the release of the corresponding peptide in the culture medium. These findings suggest that the enhanced synthesis and release of endothelin-1 occurring in conditions of increased generation of TNF, may act as a modulatory factor that counteracts the hypotensive effect and the excessive platelet aggregation and adhesion induced by TNF.

Original languageEnglish
Pages (from-to)263-266
Number of pages4
JournalMediators of Inflammation
Volume1
Issue number4
DOIs
Publication statusPublished - 1992

Keywords

  • Endothelial cells
  • Endothelin
  • Gene expression
  • Tumour necrosis factor

ASJC Scopus subject areas

  • Immunology
  • Cell Biology

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