Tumours induced by Moloney murine sarcoma virus are clonal in rats, not clonal in mice

Emma D'Andrea, Lino Tessarollo, Chiara Menin, Luigi Chieco-Bianchi

Research output: Contribution to journalArticle

Abstract

Moloney murine sarcoma virus (M-MSV) induces rapidly growing tumours in adult mice of most conventional strains. Rats are less susceptible to M-MSV oncogenesis, but the few rhabdomyosarcomas that do develop after viral inoculation of newborn animals closely resemble conventional malignancies: they develop after a long latency, grow progressively, and metastasize to regional lymph nodes and lungs. Southern blot analysis with a v-mos-specific probe of M-MSV-induced tumours in both species demonstrated an oligo-, monoclonal pattern of exogenous v-mos integration only in the rat system, while mouse tumours were not clonal in origin. Furthermore, the same type of analysis of lymph node and lung metastases showed that cell clones already present in the primary rat lesion colonized secondary sites during tumour progression. Apparently, Moloney murine leukemia virus (M-MuLV) was not involved in rhabdomyosarcoma pathogenesis since M-MuLV-specific DNA sequences could not be demonstrated in three of the six rat tumours. Finally, in all mouse tumours, unintegrated linear M-MSV proviruses could be readily detected.

Original languageEnglish
Pages (from-to)141-146
Number of pages6
JournalJournal of Cancer Research and Clinical Oncology
Volume118
Issue number2
DOIs
Publication statusPublished - Feb 1992

Keywords

  • Acute retrovirus
  • Tumour clonality
  • v-mos

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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