Tunable chemokine production by antigen presenting dendritic cells in response to changes in regulatory T cell frequency in mouse reactive lymph nodes

Valentina Dal Secco, Cristiana Soldani, Claire Debrat, François Asperti-Boursin, Emmanuel Donnadieu, Antonella Viola, Adelaida Sarukhan

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Background: Although evidence exists that regulatory T cells (Tregs) can suppress the effector phase of immune responses, it is clear that their major role is in suppressing T cell priming in secondary lymphoid organs. Recent experiments using two photon laser microscopy indicate that dendritic cells (DCs) are central to Treg cell function and that the in vivo mechanisms of T cell regulation are more complex than those described in vitro. Principal Findings: Here we have sought to determine whether and how modulation of Treg numbers modifies the lymph node (LN) microenvironment. We found that pro-inflammatory chemokines - CCL2 (MCP-1) and CCL3 (MIP-la) - are secreted in the LN early (24 h) after T cell activation, that this secretion is dependent on antigen-specific DC-T cell interactions, and that it was inversely related to the frequency of Tregs specific for the same antigen. Furthermore, we demonstrate that Tregs modify the chemoattractant properties of antigen-presenting DCs, which, as the frequency of Tregs increases, fail to produce CCL2 and CCL3 and to attract antigen-specific T cells. Conclusions: These results substantiate a major role of Tregs in LN patterning during antigen-specific immune responses.

Original languageEnglish
Article numbere7696
JournalPLoS One
Volume4
Issue number11
DOIs
Publication statusPublished - Nov 6 2009

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T-cells
antigen-presenting cells
Antigen-Presenting Cells
Regulatory T-Lymphocytes
chemokines
dendritic cells
Chemokines
Dendritic Cells
lymph nodes
T-lymphocytes
Lymph Nodes
T-Lymphocytes
Antigens
mice
antigens
immune response
Chemokine CCL2
Histocompatibility Antigens Class II
Chemotactic Factors
chemoattractants

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Tunable chemokine production by antigen presenting dendritic cells in response to changes in regulatory T cell frequency in mouse reactive lymph nodes. / Dal Secco, Valentina; Soldani, Cristiana; Debrat, Claire; Asperti-Boursin, François; Donnadieu, Emmanuel; Viola, Antonella; Sarukhan, Adelaida.

In: PLoS One, Vol. 4, No. 11, e7696, 06.11.2009.

Research output: Contribution to journalArticle

Dal Secco, Valentina ; Soldani, Cristiana ; Debrat, Claire ; Asperti-Boursin, François ; Donnadieu, Emmanuel ; Viola, Antonella ; Sarukhan, Adelaida. / Tunable chemokine production by antigen presenting dendritic cells in response to changes in regulatory T cell frequency in mouse reactive lymph nodes. In: PLoS One. 2009 ; Vol. 4, No. 11.
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