Abstract
Poly(methyl methacrylate) (PMMA) nanoparticles (NPs) were prepared by emulsion free radical polymerization. NPs with controlled dimension, as monitored by dynamic light scattering (DLS) and transmission electron microscopy (TEM), were produced by changing experimental parameters, such as the amount of emulsifier and the monomer feeding mode (batch or semi-batch). Then, different sized NPs (60, 80 and 130. nm) were loaded in polysaccharide-polyacrylic acid based hydrogels, cross-linked by covalent ester bonds between polyacrylic acid (PAA) and agarose chains, with different pore sizes (30, 60, 90. nm). The characteristics of the resulting composite hydrogel-NPs system were firstly studied in terms of rheological properties and ability to release Rhodamine B that presents steric hindrance similar to many neuroprotective agents used in spinal cord injury (SCI) repair. Then, diffusion-controlled release of different sized NPs from different entangled hydrogels was investigated in vitro and correlated with NPs diameter and hydrogel mean mesh size, showing different hindrances to the diffusion pathways. Release experiments and diffusion studies, rationalized by mathematical modeling and verified in vivo, allowed to build a material library able to satisfy different medical drug delivery needs.
| Original language | English |
|---|---|
| Pages (from-to) | 169-177 |
| Number of pages | 9 |
| Journal | Colloids and Surfaces B: Biointerfaces |
| Volume | 108 |
| DOIs | |
| Publication status | Published - Aug 1 2013 |
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Keywords
- Drug delivery
- Hydrogel
- Polymeric nanoparticles
- Release system
- Spinal cord
ASJC Scopus subject areas
- Biotechnology
- Colloid and Surface Chemistry
- Physical and Theoretical Chemistry
- Surfaces and Interfaces
Cite this
Tunable hydrogel-Nanoparticles release system for sustained combination therapies in the spinal cord. / Rossi, Filippo; Ferrari, Raffaele; Papa, Simonetta; Moscatelli, Davide; Casalini, Tommaso; Forloni, Gianluigi; Perale, Giuseppe; Veglianese, Pietro.
In: Colloids and Surfaces B: Biointerfaces, Vol. 108, 01.08.2013, p. 169-177.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Tunable hydrogel-Nanoparticles release system for sustained combination therapies in the spinal cord
AU - Rossi, Filippo
AU - Ferrari, Raffaele
AU - Papa, Simonetta
AU - Moscatelli, Davide
AU - Casalini, Tommaso
AU - Forloni, Gianluigi
AU - Perale, Giuseppe
AU - Veglianese, Pietro
PY - 2013/8/1
Y1 - 2013/8/1
N2 - Poly(methyl methacrylate) (PMMA) nanoparticles (NPs) were prepared by emulsion free radical polymerization. NPs with controlled dimension, as monitored by dynamic light scattering (DLS) and transmission electron microscopy (TEM), were produced by changing experimental parameters, such as the amount of emulsifier and the monomer feeding mode (batch or semi-batch). Then, different sized NPs (60, 80 and 130. nm) were loaded in polysaccharide-polyacrylic acid based hydrogels, cross-linked by covalent ester bonds between polyacrylic acid (PAA) and agarose chains, with different pore sizes (30, 60, 90. nm). The characteristics of the resulting composite hydrogel-NPs system were firstly studied in terms of rheological properties and ability to release Rhodamine B that presents steric hindrance similar to many neuroprotective agents used in spinal cord injury (SCI) repair. Then, diffusion-controlled release of different sized NPs from different entangled hydrogels was investigated in vitro and correlated with NPs diameter and hydrogel mean mesh size, showing different hindrances to the diffusion pathways. Release experiments and diffusion studies, rationalized by mathematical modeling and verified in vivo, allowed to build a material library able to satisfy different medical drug delivery needs.
AB - Poly(methyl methacrylate) (PMMA) nanoparticles (NPs) were prepared by emulsion free radical polymerization. NPs with controlled dimension, as monitored by dynamic light scattering (DLS) and transmission electron microscopy (TEM), were produced by changing experimental parameters, such as the amount of emulsifier and the monomer feeding mode (batch or semi-batch). Then, different sized NPs (60, 80 and 130. nm) were loaded in polysaccharide-polyacrylic acid based hydrogels, cross-linked by covalent ester bonds between polyacrylic acid (PAA) and agarose chains, with different pore sizes (30, 60, 90. nm). The characteristics of the resulting composite hydrogel-NPs system were firstly studied in terms of rheological properties and ability to release Rhodamine B that presents steric hindrance similar to many neuroprotective agents used in spinal cord injury (SCI) repair. Then, diffusion-controlled release of different sized NPs from different entangled hydrogels was investigated in vitro and correlated with NPs diameter and hydrogel mean mesh size, showing different hindrances to the diffusion pathways. Release experiments and diffusion studies, rationalized by mathematical modeling and verified in vivo, allowed to build a material library able to satisfy different medical drug delivery needs.
KW - Drug delivery
KW - Hydrogel
KW - Polymeric nanoparticles
KW - Release system
KW - Spinal cord
UR - http://www.scopus.com/inward/record.url?scp=84875791235&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84875791235&partnerID=8YFLogxK
U2 - 10.1016/j.colsurfb.2013.02.046
DO - 10.1016/j.colsurfb.2013.02.046
M3 - Article
C2 - 23537835
AN - SCOPUS:84875791235
VL - 108
SP - 169
EP - 177
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
SN - 0927-7765
ER -