Tunable hydrogel-Nanoparticles release system for sustained combination therapies in the spinal cord

Filippo Rossi, Raffaele Ferrari, Simonetta Papa, Davide Moscatelli, Tommaso Casalini, Gianluigi Forloni, Giuseppe Perale, Pietro Veglianese

Research output: Contribution to journalArticle

Abstract

Poly(methyl methacrylate) (PMMA) nanoparticles (NPs) were prepared by emulsion free radical polymerization. NPs with controlled dimension, as monitored by dynamic light scattering (DLS) and transmission electron microscopy (TEM), were produced by changing experimental parameters, such as the amount of emulsifier and the monomer feeding mode (batch or semi-batch). Then, different sized NPs (60, 80 and 130. nm) were loaded in polysaccharide-polyacrylic acid based hydrogels, cross-linked by covalent ester bonds between polyacrylic acid (PAA) and agarose chains, with different pore sizes (30, 60, 90. nm). The characteristics of the resulting composite hydrogel-NPs system were firstly studied in terms of rheological properties and ability to release Rhodamine B that presents steric hindrance similar to many neuroprotective agents used in spinal cord injury (SCI) repair. Then, diffusion-controlled release of different sized NPs from different entangled hydrogels was investigated in vitro and correlated with NPs diameter and hydrogel mean mesh size, showing different hindrances to the diffusion pathways. Release experiments and diffusion studies, rationalized by mathematical modeling and verified in vivo, allowed to build a material library able to satisfy different medical drug delivery needs.

Original languageEnglish
Pages (from-to)169-177
Number of pages9
JournalColloids and Surfaces B: Biointerfaces
Volume108
DOIs
Publication statusPublished - Aug 1 2013

Fingerprint

spinal cord
Hydrogel
Hydrogels
Nanoparticles
therapy
Spinal Cord
carbopol 940
nanoparticles
rhodamine B
Library Materials
Therapeutics
spinal cord injuries
Spinal Cord Regeneration
acids
Acids
light transmission
polysaccharides
Neuroprotective Agents
Polymethyl Methacrylate
Dynamic light scattering

Keywords

  • Drug delivery
  • Hydrogel
  • Polymeric nanoparticles
  • Release system
  • Spinal cord

ASJC Scopus subject areas

  • Biotechnology
  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry
  • Surfaces and Interfaces

Cite this

Tunable hydrogel-Nanoparticles release system for sustained combination therapies in the spinal cord. / Rossi, Filippo; Ferrari, Raffaele; Papa, Simonetta; Moscatelli, Davide; Casalini, Tommaso; Forloni, Gianluigi; Perale, Giuseppe; Veglianese, Pietro.

In: Colloids and Surfaces B: Biointerfaces, Vol. 108, 01.08.2013, p. 169-177.

Research output: Contribution to journalArticle

Rossi, Filippo ; Ferrari, Raffaele ; Papa, Simonetta ; Moscatelli, Davide ; Casalini, Tommaso ; Forloni, Gianluigi ; Perale, Giuseppe ; Veglianese, Pietro. / Tunable hydrogel-Nanoparticles release system for sustained combination therapies in the spinal cord. In: Colloids and Surfaces B: Biointerfaces. 2013 ; Vol. 108. pp. 169-177.
@article{732e386891e64d69970ce124752babe5,
title = "Tunable hydrogel-Nanoparticles release system for sustained combination therapies in the spinal cord",
abstract = "Poly(methyl methacrylate) (PMMA) nanoparticles (NPs) were prepared by emulsion free radical polymerization. NPs with controlled dimension, as monitored by dynamic light scattering (DLS) and transmission electron microscopy (TEM), were produced by changing experimental parameters, such as the amount of emulsifier and the monomer feeding mode (batch or semi-batch). Then, different sized NPs (60, 80 and 130. nm) were loaded in polysaccharide-polyacrylic acid based hydrogels, cross-linked by covalent ester bonds between polyacrylic acid (PAA) and agarose chains, with different pore sizes (30, 60, 90. nm). The characteristics of the resulting composite hydrogel-NPs system were firstly studied in terms of rheological properties and ability to release Rhodamine B that presents steric hindrance similar to many neuroprotective agents used in spinal cord injury (SCI) repair. Then, diffusion-controlled release of different sized NPs from different entangled hydrogels was investigated in vitro and correlated with NPs diameter and hydrogel mean mesh size, showing different hindrances to the diffusion pathways. Release experiments and diffusion studies, rationalized by mathematical modeling and verified in vivo, allowed to build a material library able to satisfy different medical drug delivery needs.",
keywords = "Drug delivery, Hydrogel, Polymeric nanoparticles, Release system, Spinal cord",
author = "Filippo Rossi and Raffaele Ferrari and Simonetta Papa and Davide Moscatelli and Tommaso Casalini and Gianluigi Forloni and Giuseppe Perale and Pietro Veglianese",
year = "2013",
month = "8",
day = "1",
doi = "10.1016/j.colsurfb.2013.02.046",
language = "English",
volume = "108",
pages = "169--177",
journal = "Colloids and Surfaces B: Biointerfaces",
issn = "0927-7765",
publisher = "Elsevier",

}

TY - JOUR

T1 - Tunable hydrogel-Nanoparticles release system for sustained combination therapies in the spinal cord

AU - Rossi, Filippo

AU - Ferrari, Raffaele

AU - Papa, Simonetta

AU - Moscatelli, Davide

AU - Casalini, Tommaso

AU - Forloni, Gianluigi

AU - Perale, Giuseppe

AU - Veglianese, Pietro

PY - 2013/8/1

Y1 - 2013/8/1

N2 - Poly(methyl methacrylate) (PMMA) nanoparticles (NPs) were prepared by emulsion free radical polymerization. NPs with controlled dimension, as monitored by dynamic light scattering (DLS) and transmission electron microscopy (TEM), were produced by changing experimental parameters, such as the amount of emulsifier and the monomer feeding mode (batch or semi-batch). Then, different sized NPs (60, 80 and 130. nm) were loaded in polysaccharide-polyacrylic acid based hydrogels, cross-linked by covalent ester bonds between polyacrylic acid (PAA) and agarose chains, with different pore sizes (30, 60, 90. nm). The characteristics of the resulting composite hydrogel-NPs system were firstly studied in terms of rheological properties and ability to release Rhodamine B that presents steric hindrance similar to many neuroprotective agents used in spinal cord injury (SCI) repair. Then, diffusion-controlled release of different sized NPs from different entangled hydrogels was investigated in vitro and correlated with NPs diameter and hydrogel mean mesh size, showing different hindrances to the diffusion pathways. Release experiments and diffusion studies, rationalized by mathematical modeling and verified in vivo, allowed to build a material library able to satisfy different medical drug delivery needs.

AB - Poly(methyl methacrylate) (PMMA) nanoparticles (NPs) were prepared by emulsion free radical polymerization. NPs with controlled dimension, as monitored by dynamic light scattering (DLS) and transmission electron microscopy (TEM), were produced by changing experimental parameters, such as the amount of emulsifier and the monomer feeding mode (batch or semi-batch). Then, different sized NPs (60, 80 and 130. nm) were loaded in polysaccharide-polyacrylic acid based hydrogels, cross-linked by covalent ester bonds between polyacrylic acid (PAA) and agarose chains, with different pore sizes (30, 60, 90. nm). The characteristics of the resulting composite hydrogel-NPs system were firstly studied in terms of rheological properties and ability to release Rhodamine B that presents steric hindrance similar to many neuroprotective agents used in spinal cord injury (SCI) repair. Then, diffusion-controlled release of different sized NPs from different entangled hydrogels was investigated in vitro and correlated with NPs diameter and hydrogel mean mesh size, showing different hindrances to the diffusion pathways. Release experiments and diffusion studies, rationalized by mathematical modeling and verified in vivo, allowed to build a material library able to satisfy different medical drug delivery needs.

KW - Drug delivery

KW - Hydrogel

KW - Polymeric nanoparticles

KW - Release system

KW - Spinal cord

UR - http://www.scopus.com/inward/record.url?scp=84875791235&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84875791235&partnerID=8YFLogxK

U2 - 10.1016/j.colsurfb.2013.02.046

DO - 10.1016/j.colsurfb.2013.02.046

M3 - Article

C2 - 23537835

AN - SCOPUS:84875791235

VL - 108

SP - 169

EP - 177

JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

SN - 0927-7765

ER -